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Literature summary extracted from

  • Yoon, G.; Ko, K.; Kang, H.; Suh, J.; Kim, Y.; Ryu, Y.
    Characterization of S-adenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production (2006), Enzyme Microb. Technol., 39, 466-473.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 gene SAM-s, DNA and amino acid sequence determination and analysis, a putative purine-rich ribosomal binding site is located 3 bp upstream of the GTG start codon, potential -35 and -10 consensus promoter sequences, expression of His-tagged enzyme in Escherichia coli strain XL-1 Blue, overexpression in Streptomyces avermitilis leads to 2fold increased synthesis of the antibiotic avermectin, overexpression in Streptomyces peucetius leads to 3.5fold increased synthesis of the antibiotic doxorubicin Streptomyces avermitilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.11
-
 L-methionine pH 8.0, 37°C Streptomyces avermitilis
2.5.1.6 0.34
-
 ATP pH 8.0, 37°C Streptomyces avermitilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 K+ bound by G49 Streptomyces avermitilis
2.5.1.6 Mg2+ the enzyme requires Mn2+ or Mg2+, which are equally active, bound by D17 and D289 Streptomyces avermitilis
2.5.1.6 Mn2+ the enzyme requires Mn2+ or Mg2+, which are equally active Streptomyces avermitilis
2.5.1.6 additional information Ag+, Ca2+, Fe2+, and Cu2+ cannot substitue for Mg2+ or Mn2+ Streptomyces avermitilis
2.5.1.6 Zn2+ 66% of the activity with Mg2+ or Mn2+ Streptomyces avermitilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 43500
-
 x * 43500, recombinant enzyme, SDS-PAGE Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O Streptomyces avermitilis
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
2.5.1.6 ATP + L-methionine + H2O Streptomyces avermitilis NRRL 8165
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Streptomyces avermitilis Q3HW35 gene SAM-s
-
2.5.1.6 Streptomyces avermitilis NRRL 8165 Q3HW35 gene SAM-s
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 recombinant His-tagged enzyme from Escherichia coli strain XL-1 Blue by nickel affiniy chromatography Streptomyces avermitilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.6 mycelium
-
 Streptomyces avermitilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.6 additional information
-
-
 Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
 Streptomyces avermitilis phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
2.5.1.6 ATP + L-methionine + H2O
-
 Streptomyces avermitilis NRRL 8165 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 ? x * 43500, recombinant enzyme, SDS-PAGE Streptomyces avermitilis
2.5.1.6 More the enzyme contains conserved sequence motifs of SAM-s proteins, such as nanopeptide 276GGGAFSGKD284 for the P-loop for ATP binding site and metal binding site with 17D and 289D for Mg2+ and 49G for K+ Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.6 S-adenosylmethionine synthetase
-
 Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 35
-
-
 Streptomyces avermitilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.6 20 50
-
 Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.6 8
-
-
 Streptomyces avermitilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.6 5 10
-
 Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.6 ATP the binding site contains the conserved nanopeptide 276GGGAFSGKD284 for the P-loop Streptomyces avermitilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.6 Streptomyces avermitilis sequence calculation
-
 4.82