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Literature summary extracted from

  • Pai, C.H.; Chiang, B.Y.; Ko, T.P.; Chou, C.C.; Chong, C.M.; Yen, F.J.; Chen, S.; Coward, J.K.; Wang, A.H.; Lin, C.H.
    Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase (2006), EMBO J., 25, 5970-5982.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.8 expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.8 hanging drop vapour diffusion method generating crystals of GspS in 0.1M Tris–HCl (pH 8.5) containing 12% (v/v) PEG3350 and 0.5 M MgCl2 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.8 C338A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 E391A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 E392A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 K607A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 R316E no activity Escherichia coli
6.3.1.8 R538A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 R598A decreased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 S335A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 S337A increased Km compared to the wild type enzyme Escherichia coli
6.3.1.8 T441A increased Km compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.8 (2-[[([2-[(4-amino-4-carboxybutanoyl)amino]propanoyl]amino)methyl](hydroxy)phosphoryl]ethyl)(hydroxy)dioxophosphate(1-) inhibits the reaction in the presence of spermidine Escherichia coli
6.3.1.8 4-[(14-ammonio-5-hydroxy-1-methyl-5-oxido-2-oxo-6-oxa-3,10-diaza-5-phosphatetradec-1-yl)amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli
6.3.1.8 4-[[2-([[[4-[(4-ammoniobutyl)amino]butyl](hydroxy)phosphoryl]methyl]amino)-1-methyl-2-oxoethyl]amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli
6.3.1.8 gamma-glutamyl-N-[[(3-aminopropoxy)(2-phosphonoethyl)phosphoryl]methyl]alaninamide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.8 0.0224
-
 glutathione mutant enzyme R598A Escherichia coli
6.3.1.8 0.076
-
 spermidine wild type enzyme Escherichia coli
6.3.1.8 0.076
-
 spermidine mutant enzyme S337A Escherichia coli
6.3.1.8 0.126
-
 spermidine mutant enzyme S335A Escherichia coli
6.3.1.8 0.153
-
 spermidine mutant enzyme K607A Escherichia coli
6.3.1.8 0.182
-
 spermidine mutant enzyme R538A Escherichia coli
6.3.1.8 0.218
-
 glutathione wild type enzyme Escherichia coli
6.3.1.8 0.224
-
 glutathione mutant enzyme S337A Escherichia coli
6.3.1.8 0.251
-
 glutathione mutant enzyme K607A Escherichia coli
6.3.1.8 0.406
-
 spermidine mutant enzyme C338A Escherichia coli
6.3.1.8 0.407
-
 glutathione mutant enzyme E391A Escherichia coli
6.3.1.8 0.665
-
 spermidine mutant enzyme R598A Escherichia coli
6.3.1.8 0.691
-
 glutathione mutant enzyme T441A Escherichia coli
6.3.1.8 0.794
-
 glutathione mutant enzyme S335A Escherichia coli
6.3.1.8 1.008
-
 spermidine mutant enzyme E392A Escherichia coli
6.3.1.8 1.324
-
 glutathione mutant enzyme R538A Escherichia coli
6.3.1.8 1.61
-
 spermidine mutant enzyme T441A Escherichia coli
6.3.1.8 2.098
-
 glutathione mutant enzyme C338A Escherichia coli
6.3.1.8 4.522
-
 spermidine mutant enzyme E391A Escherichia coli
6.3.1.8 7.298
-
 glutathione mutant enzyme E392A Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.8 Mg2+ two Mg ions per molecule Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.8 70000
-
 2 * 70000, analytical ultracentrifugation Escherichia coli
6.3.1.8 138000
-
 analytical ultracentrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Escherichia coli P0AES0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.8 nickel affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.8 glutathione + spermidine + ATP
-
 Escherichia coli glutathionylspermidine + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.8 dimer 2 * 70000, analytical ultracentrifugation Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.1.8 glutathionylspermidine synthetase
-
 Escherichia coli
6.3.1.8 GspS
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.8 ATP
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.1.8 0.0000078
-
 4-[[2-([[[4-[(4-ammoniobutyl)amino]butyl](hydroxy)phosphoryl]methyl]amino)-1-methyl-2-oxoethyl]amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli
6.3.1.8 0.006
-
 4-[(14-ammonio-5-hydroxy-1-methyl-5-oxido-2-oxo-6-oxa-3,10-diaza-5-phosphatetradec-1-yl)amino]-1-carboxy-4-oxobutan-1-aminium
-
 Escherichia coli