Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ulusu, N.N.; Tandogan, B.
    Purification and kinetics of sheep kidney cortex glucose-6-phosphate dehydrogenase (2006), Comp. Biochem. Physiol. B, 143B, 249-255.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 additional information
-
 additional information ping pong bi bi kinetic mechanism Ovis aries
1.1.1.49 0.0147
-
 NADP+ pH 7.4, 37°C Ovis aries
1.1.1.49 0.041
-
 D-glucose 6-phosphate pH 7.4, 37°C Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.49 cytosol
-
 Ovis aries 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ + H2O Ovis aries the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals D-gluconate 6-phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 native enzyme 1384fold from kidney cortex by ultracentrifugation, 2',5'-ADP affinity and anion exchange chromatography Ovis aries

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.49 D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ the adult kidney cortex enzyme shows a ping pong bi bi kinetic mechanism, while the lamb kidney enzyme obeys an ordered bi bi mechanism, thus the kinetic mechanism alters due to aging Ovis aries
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.49 kidney cortex
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.49 27.69
-
 purified kidney cortex enzyme Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ + H2O
-
 Ovis aries D-gluconate 6-phosphate + NADPH + H+
-
 ?
1.1.1.49 D-glucose 6-phosphate + NADP+ + H2O the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals Ovis aries D-gluconate 6-phosphate + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G-6-PD
-
 Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.49 45
-
-
 Ovis aries

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.49 20 60 temperature profile Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 7.4
-
-
 Ovis aries

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.49 5 10 pH profile Ovis aries

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.49 6.5 10 stable, purified enzyme Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 Ovis aries