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Literature summary extracted from
- A regioselective tryptophan 5-halogenase is involved in pyrroindomycin biosynthesis in Streptomyces rugosporus LL-42D005 (2005), Chem. Biol., 12, 445-452.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.58 | overexpression in Pseudomonas fluorescens BL915 DELTAORF1 | Streptomyces rugosporus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.58 | 0.15 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces rugosporus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | Streptomyces rugosporus | the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | Streptomyces rugosporus LL-42D005 | the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.58 | Streptomyces rugosporus | A4D0H5 | - |
- |
| 1.14.19.58 | Streptomyces rugosporus LL-42D005 | A4D0H5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.58 | - |
Streptomyces rugosporus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.58 | L-tryptophan + FADH2 + Br- + O2 + H+ | brominating activity is about 75% of the chlorinating activity | Streptomyces rugosporus | 5-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + Br- + O2 + H+ | brominating activity is about 75% of the chlorinating activity | Streptomyces rugosporus LL-42D005 | 5-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Streptomyces rugosporus | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position | Streptomyces rugosporus | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Streptomyces rugosporus LL-42D005 | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
| 1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position | Streptomyces rugosporus LL-42D005 | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.58 | pyrH | - |
Streptomyces rugosporus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.58 | 30 | - |
assay at | Streptomyces rugosporus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.58 | 0.00813 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces rugosporus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.58 | 7.2 | - |
assay at | Streptomyces rugosporus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.58 | malfunction | a gene disruption mutant in the tryptophan 5-halogenase gene no longer produces the pyrroindomycin B, but still produces pyrroindomycin A, the nonhalogenated derivative | Streptomyces rugosporus |
| 1.14.19.58 | physiological function | the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position | Streptomyces rugosporus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.58 | 0.0542 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces rugosporus | |
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