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Literature summary extracted from

  • Haraga, A.; Miller, S.I.
    A Salmonella type III secretion effector interacts with the mammalian serine/threonine protein kinase PKN1 (2006), Cell. Microbiol., 8, 837-846.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 fatty acids activate PKN1 Homo sapiens
2.7.11.1 Rho activates PKN1 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expression of FLAG-tagged full-length PKN1 and of its isolated catalytic kinase domain, residues 561-942, in CHO-K1 cells, co-expression with Salmonella enterica SspH1, SspH2, YopM and IpaH9.8, all HA-tagged, in HeLa cells, interaction study, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 K644E site-directed mutagenesis, inactive mutant, PKN1 depletion causes an increase in NF-kappaB-dependent reporter gene expression Homo sapiens
2.7.11.1 additional information PKN1 depletion by siRNA causes an increase in NF-kappaB-dependent reporter gene expression Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 additional information Homo sapiens the enzyme inhibits NF-kappaB activity via interaction with the leucine-rich repeat domain of the Salmonella enterica serovar typhimurium-derived effector SspH1 in infected intestine-407 cells, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein phosphorylation at Thr774 of the activation loop is required for activity in vivo, PKN1 is phosphorylated during Salmonella enterica infection independent of SspH1 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 intestine-407 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 additional information the enzyme inhibits NF-kappaB activity via interaction with the leucine-rich repeat domain of the Salmonella enterica serovar typhimurium-derived effector SspH1 in infected intestine-407 cells, overview Homo sapiens ?
-
 ?
2.7.11.1 additional information the catalytic kinase domain, residues 561-942, of PKN1 is active with out the regulatory domain Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PKN1
-
 Homo sapiens
2.7.11.1 protein kinase N1
-
 Homo sapiens
2.7.11.1 serine/threonine protein kinase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Homo sapiens