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Literature summary extracted from

  • Blasius, M.; Buob, R.; Shevelev, I.V.; Hubscher, U.
    Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans (2007), BMC Mol. Biol., 8, 69.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 K128A mutant enzyme shows no ligation activity Deinococcus radiodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 ATP 1 mM Deinococcus radiodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.2 0.105
-
 nicked DNA
-
 Deinococcus radiodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Ca2+ 10fold lower activation than Mn2+, maximal activity at 2 mM Deinococcus radiodurans
6.5.1.2 Mg2+ 10fold lower activation than Mn2+, maximal activity at 1 mM Deinococcus radiodurans
6.5.1.2 Mn2+ preference for Mn2+ as cofactor, maximal activity at 1 mM Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Deinococcus radiodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 recombinant Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 ATP + nicked DNA
-
 Deinococcus radiodurans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.2 30
-
-
 Deinococcus radiodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.2 6.8
-
-
 Deinococcus radiodurans