EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.3 | F42C | mutant retains more than 70% of activity after 1 h, while the wild-type enzyme retains only 10% activity. Optimal temperature of mutant enzyme is about 10°C higher than that of wild-type enzyme. Activity at the optimal temperature is about 20% higher than that of wild-type enzyme. KM-values for D-Ala, D-Met, D-Phe and D-Ser are 40-50% of the wild-type value | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 0.4 | - |
D-Met | pH 8.3, 37°C, mutant enzyme F42C | Sus scrofa | |
1.4.3.3 | 0.9 | - |
D-Met | pH 8.3, 37°C, wild-type enzyme | Sus scrofa | |
1.4.3.3 | 1.2 | - |
D-Ala | pH 8.3, 37°C, mutant enzyme F42C | Sus scrofa | |
1.4.3.3 | 1.9 | - |
D-Phe | pH 8.3, 37°C, mutant enzyme F42C | Sus scrofa | |
1.4.3.3 | 2.5 | - |
D-Ala | pH 8.3, 37°C, wild-type enzyme | Sus scrofa | |
1.4.3.3 | 3.6 | - |
D-Phe | pH 8.3, 37°C, wild-type enzyme | Sus scrofa | |
1.4.3.3 | 4.7 | - |
D-Ser | pH 8.3, 37°C, mutant enzyme F42C | Sus scrofa | |
1.4.3.3 | 12.7 | - |
D-Ser | pH 8.3, 37°C, wild-type enzyme | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Sus scrofa | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.3 | kidney | - |
Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | D-Ala + H2O + O2 | - |
Sus scrofa | pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Met + H2O + O2 | - |
Sus scrofa | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Phe + H2O + O2 | - |
Sus scrofa | phenylpyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Ser + H2O + O2 | - |
Sus scrofa | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 45 | 50 | wild-type enzyme | Sus scrofa |
1.4.3.3 | 55 | - |
mutant enzyme F42C | Sus scrofa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 30 | 60 | 30°C: about 50% of maximal activity, 60°C: about 40% of maximal activity, wild-type enzyme | Sus scrofa |
1.4.3.3 | 40 | 70 | 40°C and 70°C: about 60% of maximal activity, mutant enzyme F42C | Sus scrofa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 45 | - |
wild-type enzyme is unstable above | Sus scrofa |
1.4.3.3 | 55 | - |
1 h, mutant enzyme F42C retains more than 70% of activity, wild-type enzyme retains only 10% activity | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 9 | 10 | wild-type enzyme and mutant enzyme F42C | Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 7.5 | 11 | pH 7.5 and pH 11: about 40% of maximal activity | Sus scrofa |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 5.5 | 9 | 25°C, 24 h, stable, wild-type enzyme and mutant enzyme F42C | Sus scrofa |