EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.1.84 | x-ray diffraction, special focus on iron center vicinity and hydrogen bond network, similar structures of wild type and alphaQ90N mutant enzyme in nitrosylated state, resolution: 1.43 A | Rhodococcus sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.84 | Q90E | alpha subunit | Rhodococcus sp. |
4.2.1.84 | Q90N | alpha subunit | Rhodococcus sp. |
4.2.1.84 | R56K | beta subunit | Rhodococcus sp. |
EC Number | General Stability | Organism |
---|---|---|
4.2.1.84 | purified mutant NHases are stored in the dark without n-butyric acid, before use, NHases are activated by light irradiation. | Rhodococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.84 | n-butyric acid | - |
Rhodococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.84 | 0.28 | - |
Methacrylonitrile | mutant alphaQ90E, vmax: 280 micromol/min/mg | Rhodococcus sp. | |
4.2.1.84 | 0.68 | - |
Methacrylonitrile | wild type, vmax: 1200 micromol/min/mg | Rhodococcus sp. | |
4.2.1.84 | 1.9 | - |
Methacrylonitrile | mutant betaR56K, vmax: 2.5 micromol/min/mg | Rhodococcus sp. | |
4.2.1.84 | 2.6 | - |
Methacrylonitrile | mutant alphaQ90N, vmax: 57 micromol/min/mg | Rhodococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.84 | Rhodococcus sp. | - |
- |
- |
4.2.1.84 | Rhodococcus sp. N771 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.84 | 3 successive column chromatographies in the presence of 40 mM n-butyric acid (only wild type), alphaQ90N mutein is purified in nitrosylated state in the dark | Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.84 | methacrylonitrile + H2O | - |
Rhodococcus sp. | methacrylamide | - |
? | |
4.2.1.84 | methacrylonitrile + H2O | - |
Rhodococcus sp. N771 | methacrylamide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.84 | NHase | - |
Rhodococcus sp. |
4.2.1.84 | nitrile hydratase | - |
Rhodococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.84 | 120 | - |
Methacrylonitrile | mutant betaR56K | Rhodococcus sp. | |
4.2.1.84 | 2600 | - |
Methacrylonitrile | mutant alphaQ90N | Rhodococcus sp. | |
4.2.1.84 | 13000 | - |
Methacrylonitrile | mutant alphaQ90E | Rhodococcus sp. | |
4.2.1.84 | 55000 | - |
Methacrylonitrile | wild type | Rhodococcus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.84 | 2.6 | - |
Butyric acid | +/- 0.2, substrate: methacrylonitrile, mutant betaR56K | Rhodococcus sp. | |
4.2.1.84 | 5.1 | - |
Butyric acid | +/- 0.3, substrate: methacrylonitrile, mutant alphaQ90E | Rhodococcus sp. | |
4.2.1.84 | 6.2 | - |
Butyric acid | +/- 0.9, substrate: methacrylonitrile, wild type | Rhodococcus sp. | |
4.2.1.84 | 14 | - |
Butyric acid | +/- 2.7, substrate: methacrylonitrile, mutant alphaQ90N | Rhodococcus sp. |