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Literature summary extracted from

  • Suenaga, H.; Sato, M.; Goto, M.; Takeshita, M.; Furukawa, K.
    Steady-state kinetic characterization of evolved biphenyl dioxygenase, which acquired novel degradation ability for benzene and toluene (2006), Biosci. Biotechnol. Biochem., 70, 1021-1025.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 expressed in Escherichia coli Pseudomonas oleovorans
1.14.12.18 expressed in Escherichia coli Paraburkholderia xenovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.18 0.0027
-
 biphenyl Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.00342
-
 biphenyl Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans
1.14.12.18 0.00368
-
 biphenyl Pseudomonas pseudoalcaligenes strain LB400 Pseudomonas oleovorans
1.14.12.18 0.00433
-
 Benzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.0108
-
 ethylbenzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.0117
-
 Toluene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.0236
-
 ethylbenzene Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Paraburkholderia xenovorans
-
-
-
1.14.12.18 Pseudomonas oleovorans
-
 strains KF707 and 1072
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.18 Q-Sepharose FF column chromatography Pseudomonas oleovorans
1.14.12.18 Q-Sepharose FF column chromatography Paraburkholderia xenovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 benzene + ? benzene is only a substrate for Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans ?
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Paraburkholderia xenovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 ethylbenzene + ?
-
 Pseudomonas oleovorans ?
-
 ?
1.14.12.18 toluene + NAD(P)H + O2 toluene is only a substrate for Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 biphenyl dioxygenase
-
 Pseudomonas oleovorans
1.14.12.18 biphenyl dioxygenase
-
 Paraburkholderia xenovorans
1.14.12.18 BPH dox
-
 Pseudomonas oleovorans
1.14.12.18 BPH dox
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.12.18 0.0497
-
 ethylbenzene Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans
1.14.12.18 0.056
-
 Benzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.113
-
 biphenyl Pseudomonas pseudoalcaligenes strain KF707 Pseudomonas oleovorans
1.14.12.18 0.114
-
 biphenyl Pseudomonas pseudoalcaligenes strain LB400 Pseudomonas oleovorans
1.14.12.18 0.115
-
 Toluene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.168
-
 biphenyl Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans
1.14.12.18 0.219
-
 ethylbenzene Pseudomonas pseudoalcaligenes strain 1072 Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 NADH
-
 Pseudomonas oleovorans
1.14.12.18 NADH
-
 Paraburkholderia xenovorans
1.14.12.18 NADPH
-
 Pseudomonas oleovorans
1.14.12.18 NADPH
-
 Paraburkholderia xenovorans