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Literature summary extracted from

  • Vintem, A.P.; Price, N.T.; Silverman, R.B.; Ramsay, R.R.
    Mutation of surface cysteine 374 to alanine in monoamine oxidase A alters substrate turnover and inactivation by cyclopropylamines (2005), Bioorg. Med. Chem., 13, 3487-3495.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.4 expression of wild-type MAO A and mutant MAO A C374A in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.4 C374A mutation of surface cysteine 374 to alanine alters substrate turnover and inactivation by cyclopropylamines Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 (N-cyclopropyl-alpha-methylbenzylamine) the inhibitor forms an adduct that allows reoxidation of the flavin on denaturation Homo sapiens
1.4.3.4 1-methyl-4-phenylpyridinium
-
 Homo sapiens
1.4.3.4 1-phenylcyclopropylamine the inhibitor forms an irreversible flavin adduct Homo sapiens
1.4.3.4 2-phenylcyclopropylamine the inhibitor forms an adduct that allows reoxidation of the flavin on denaturation Homo sapiens
1.4.3.4 D-amphetamine
-
 Homo sapiens
1.4.3.4 harman
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 0.033
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.039
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.1
-
 kynuramine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.17
-
 kynuramine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.42
-
 benzylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.43
-
 serotonin pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.44
-
 Phenylethylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.49
-
 Phenylethylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.51
-
 serotonin pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.61
-
 benzylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Homo sapiens P21397
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.4 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine + H2O + O2
-
 Homo sapiens ?
-
 ?
1.4.3.4 benzylamine + H2O + O2
-
 Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 kynuramine + H2O + O2
-
 Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
1.4.3.4 phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 serotonin + H2O + O2
-
 Homo sapiens (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO A
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.4 0.079
-
 benzylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.09
-
 benzylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.241
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.29
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 2.2
-
 kynuramine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 2.5
-
 Phenylethylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 2.5
-
 kynuramine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 3.1
-
 Phenylethylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 16
-
 serotonin pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 18.6
-
 serotonin pH 7.2, 30°C, wild-type enzyme Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.4 0.000128
-
 harman pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.00013
-
 harman pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.0035
-
 1-methyl-4-phenylpyridinium pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.0045
-
 1-methyl-4-phenylpyridinium pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.0115
-
 D-amphetamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 0.0144
-
 D-amphetamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.2
-
 2-phenylcyclopropylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 0.31
-
 2-phenylcyclopropylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 1.4
-
 (N-cyclopropyl-alpha-methylbenzylamine) pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 2.7
-
 (N-cyclopropyl-alpha-methylbenzylamine) pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4.3.4 7.3
-
 1-phenylcyclopropylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
1.4.3.4 12.8
-
 1-phenylcyclopropylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens