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Literature summary extracted from

  • Reda, M.; Klobus, G.
    Modifications of the activity of nitrate reductase from cucumber roots (2006), Biol. Plant., 50, 42-47.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 ATP at low pH, the presence of ATP alone in the incubation medium is sufficient to inactivate nitrate reductase Cucumis sativus
1.7.1.1 Mg2+ 5 mM MgCl2 decreases enzyme activity at pH 7.5, this effect is completely reversed by the addition of EDTA into samples returning the enzyme's activity to its initial level Cucumis sativus
1.7.1.1 additional information An inhibitory effect of Mg-ATP is cancelled in the presence of staurosporine (the protein kinase inhibitor) and completely reversed after addition of EDTA as well as AMP Cucumis sativus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.1 EDTA 5 mM, 27% increase of activity at pH 7.5 Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Cucumis sativus
-
 cultivar Wisconsin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1 Sephadex G-25 gel filtration Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 root
-
 Cucumis sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH
-
 Cucumis sativus nitrite + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.1 NADH-dependent nitrate reductase
-
 Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 7.5
-
-
 Cucumis sativus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.1 additional information
-
 activity determined at pH 6.0 was significantly lower than at pH 7.5 Cucumis sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Cucumis sativus