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Literature summary extracted from

  • Kopecny, D.; Pethe, C.; Sebela, M.; Houba-Herin, N.; Madzak, C.; Majira, A.; Laloue, M.
    High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica (2005), Biochimie, 87, 1011-1022.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 high-level expression of cytokinin oxidase/dehydrogenase in Yarrowia lipolytica Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.12 0.0011
-
 N6-isopentenyladenine 30°C, pH 7.4 Zea mays
1.5.99.12 0.009
-
 zeatin 30°C, pH 7.4 Zea mays
1.5.99.12 0.012
-
 N6-isopentenyladenosine 30°C, pH 7.4 Zea mays
1.5.99.12 0.063
-
 zeatin riboside 30°C, pH 7.4 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.12 5600
-
 1 * 5600, deglycosylated enzyme, SDS-PAGE Zea mays
1.5.99.12 64000
-
 gel filtration, MALDI-MS Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Zea mays Q9T0N8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.99.12 glycoprotein contains 22% neutral sugars Zea mays

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.12
-
 Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.12 62.4
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 N6-isopentenyladenine + 2,6-dichlorophenolindophenol
-
 Zea mays adenine + 3-methyl-2-butenal + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.12 N6-isopentenyladenosine + 2,6-dichlorophenolindophenol
-
 Zea mays 3-methylbut-2-enal + adenosine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.12 zeatin + 2,6-dichlorophenolindophenol
-
 Zea mays 4-hydroxy-3-methylbut-2-enal + adenine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.12 zeatin riboside + 2,6-dichlorophenolindophenol
-
 Zea mays ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.12 monomer 1 * 5600, deglycosylated enzyme, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 cytokinin oxidase/dehydrogenase
-
 Zea mays
1.5.99.12 ZmCKO1
-
 Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.99.12 55
-
 T50 for 30 min incubation Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.99.12 21
-
 zeatin riboside 30°C, pH 7.4 Zea mays
1.5.99.12 62
-
 N6-isopentenyladenosine 30°C, pH 7.4 Zea mays
1.5.99.12 128
-
 N6-isopentenyladenine 30°C, pH 7.4 Zea mays
1.5.99.12 135
-
 zeatin 30°C, pH 7.4 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.12 7
-
 reaction withz zeatin derivatives Zea mays
1.5.99.12 7.2
-
 reaction with N6-(2-isopentenyl)adenine derivatives Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 FAD
-
 Zea mays

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.99.12 Zea mays calculated from sequence
-
 5.9
1.5.99.12 Zea mays chromatofocusing
-
 6.25