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Literature summary extracted from
- Biochemical and functional characterization of UDP-galactose 4-epimerase from Aeromonas hydrophila (2007), Biochim. Biophys. Acta, 1774, 828-837.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.1.3.2 | medicine | drug design | Aeromonas hydrophila |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.2 | expression in Escherichia coli | Aeromonas hydrophila |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.2 | 0.5 | - |
UDP-galactose | - |
Aeromonas hydrophila |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 37000 | - |
MALDI-TOF MS | Aeromonas hydrophila |
| 5.1.3.2 | 38270 | - |
MALDI-TOF MS | Aeromonas hydrophila |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.2 | additional information | Aeromonas hydrophila | Leloir pathway of galactose metabolism | ? | - |
? | |
| 5.1.3.2 | UDP-galactose | Aeromonas hydrophila | - |
UDP-glucose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.2 | Aeromonas hydrophila | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.2 | DEAE-Sepharose anion exchange chromatography | Aeromonas hydrophila |
| 5.1.3.2 | Ni2+-NTA affinity chromatography | Aeromonas hydrophila |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.2 | additional information | Leloir pathway of galactose metabolism | Aeromonas hydrophila | ? | - |
? | |
| 5.1.3.2 | UDP-galactose | - |
Aeromonas hydrophila | UDP-glucose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.2 | monomer | - |
Aeromonas hydrophila |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.2 | 6xHis-rGalE | - |
Aeromonas hydrophila |
| 5.1.3.2 | GalE | - |
Aeromonas hydrophila |
| 5.1.3.2 | rGalE | - |
Aeromonas hydrophila |
| 5.1.3.2 | UDP-galactose 4-epimerase | - |
Aeromonas hydrophila |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 37 | - |
- |
Aeromonas hydrophila |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 25 | 45 | - |
Aeromonas hydrophila |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.2 | 12.9 | - |
UDP-galactose | - |
Aeromonas hydrophila | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 7 | - |
- |
Aeromonas hydrophila |
| 5.1.3.2 | 8 | 9 | - |
Aeromonas hydrophila |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 3 | 10 | - |
Aeromonas hydrophila |
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Release 2023.1 (January 2023)
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