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Literature summary extracted from

  • Yan, Y.; Nguyen, H.; Dalmasso, G.; Sitaraman, S.V.; Merlin, D.
    Cloning and characterization of a new intestinal inflammation-associated colonic epithelial Ste20-related protein kinase isoform (2007), Biochim. Biophys. Acta, 1769, 106-116.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 additional information expression of the intestinal inflammation-associated colonic epithelial SPAK isozyme is upregulated by the pro-inflammatory cytokine IFN-gamma, but not by TGF-beta and TNF-alpha, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 DNA and amino acid sequence determination and analysis, in vitro transcription and translation, overexpression of the SPAK isozyme in Caco-2-BBE cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 I34L site-directed mutagenesis, inactive mutant of the intestinal inflammation-associated colonic epithelial isozyme Homo sapiens
2.7.11.1 additional information overexpression of the SPAK isozyme leads to leading to nuclear translocation of an N-terminal fragment of the SPAK isozyme, as well as activation of p38 MAP kinase signaling cascades and increased intestinal barrier permeability Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 52600
-
 x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE Homo sapiens
2.7.11.1 53000
-
 x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE Homo sapiens
2.7.11.1 60000
-
 x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 additional information Homo sapiens the enzyme is involved in the inflammation cascade in intestine and colon epithelium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Homo sapiens Q6E0B2 small intestine SPAK-like kinase; intestinal inflammation-associated colonic epithelial isozyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 colon epithelium, inflamed and non-inflamed tissue Homo sapiens
-
2.7.11.1 epithelium
-
 Homo sapiens
-
2.7.11.1 intestine
-
 Homo sapiens
-
2.7.11.1 additional information no activity of the intestinal inflammation-associated colonic epithelial isozyme in liver, spleen, brain, prostate, and kidney Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + myelin basic protein
-
 Homo sapiens ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.1 additional information the enzyme is involved in the inflammation cascade in intestine and colon epithelium Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 ? x * 52600, about, sequence calculation, x * 53000, in vitro expressed enzyme, SDS-PAGE, x * 60000, about, enzyme expressed in Caco-2-BBE cells, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 serine/threonine kinase
-
 Homo sapiens
2.7.11.1 SPAK/STE20
-
 Homo sapiens
2.7.11.1 SPS1-related proline alanine-rich kinase
-
 Homo sapiens
2.7.11.1 Ste20-related protein kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.1 Homo sapiens sequence calculation
-
 6.51