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Literature summary extracted from

  • Orland, M.D.; Anwar, K.; Cromley, D.; Chu, C.; Chen, L.; Billheimer, J.T.; Hussain, M.M.; Cheng, D.
    Acyl coenzyme A dependent retinol esterification by acyl coenzyme A:diacylglycerol acyltransferase 1 (2005), Biochim. Biophys. Acta, 1737, 76-82.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.76 expression in Spodoptera frugiperda Sf9 cell membranes using the baculovrisu transfection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.22 XP620 selective isozyme DGAT1 inhibitor, inhibits the diacylglycerol acyltransferase and the retinol acyltransferase activities at the retinol recognition site Homo sapiens
2.3.1.76 XP620 inhibits the ARAT activity of acyl coenzyme A:diacylglycerol acyltransferase isozyme 1 dependent on the retinol concentration in vivo and in vitro, IC50: 110 nM, no inhibition of isozyme 2 and acyl coenzyme A:monoacylglycerol acyltransferase isozyme 3, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.76 0.0139
-
 palmitoyl-CoA ARAT activity of recombinant acyl coenzyme A:diacylglycerol acyltransferase isozyme 1, pH 7.4, 37°C Homo sapiens
2.3.1.76 0.0259
-
 retinol ARAT activity of recombinant acyl coenzyme A:diacylglycerol acyltransferase isozyme 1, pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane
-
 Homo sapiens 16020
-
2.3.1.20 microsome
-
 Homo sapiens
-
-
2.3.1.22 membrane
-
 Homo sapiens 16020
-
2.3.1.22 microsome
-
 Homo sapiens
-
-
2.3.1.76 membrane
-
 Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 2-monoacylglycerol Homo sapiens
-
 CoA + diacylglycerol
-
 ?
2.3.1.22 acyl-CoA + 1,2-diacylglycerol Homo sapiens
-
 CoA + triacylglycerol
-
 ?
2.3.1.76 acyl-CoA + retinol Homo sapiens
-
 CoA + retinyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Homo sapiens
-
-
-
2.3.1.22 Homo sapiens
-
 isozyme DGAT1
-
2.3.1.76 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 Caco-2 cell
-
 Homo sapiens
-
2.3.1.22 Caco-2 cell
-
 Homo sapiens
-
2.3.1.76 Caco-2 cell colon cancer cell line Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.76 0.00045
-
 ARAT activity of recombinant acyl coenzyme A:diacylglycerol acyltransferase isozyme 2 in SF9 cell membrane preparation Homo sapiens
2.3.1.76 0.00084
-
 ARAT activity of recombinant acyl coenzyme A:monoacylglycerol acyltransferase isozyme 3 in SF9 cell membrane preparation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 2-monoacylglycerol
-
 Homo sapiens CoA + diacylglycerol
-
 ?
2.3.1.20 additional information the enzyme also performs the reaction of the retinol O-fatty-acyltransferase, EC 2.3.1.76 Homo sapiens ?
-
 ?
2.3.1.22 acyl-CoA + 1,2-diacylglycerol
-
 Homo sapiens CoA + triacylglycerol
-
 ?
2.3.1.22 additional information isozyme DGAT1 also performs the reaction of the retinol O-fatty-acyltransferase, EC 2.3.1.76 Homo sapiens ?
-
 ?
2.3.1.76 acyl-CoA + retinol
-
 Homo sapiens CoA + retinyl ester
-
 ?
2.3.1.76 additional information acyl coenzyme A:diacylglycerol acyltransferase, DGAT, EC 2.3.1.20, and acyl coenzyme A:monoacylglycerolol acyltransferase, MGAT, EC 2.3.1.22, also show activity with retinol, overview Homo sapiens ?
-
 ?
2.3.1.76 palmitoyl-CoA + retinol
-
 Homo sapiens CoA + retinyl palmitate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 acyl coenzyme A:diacylglycerol acyltransferase 1
-
 Homo sapiens
2.3.1.20 MGAT
-
 Homo sapiens
2.3.1.20 More cf. EC 2.3.1.76 Homo sapiens
2.3.1.22 acyl coenzyme A:diacylglycerol acyltransferase
-
 Homo sapiens
2.3.1.22 DGAT
-
 Homo sapiens
2.3.1.22 DGAT1/ARAT
-
 Homo sapiens
2.3.1.22 More cf. EC 2.3.1.76 Homo sapiens
2.3.1.76 acyl coenzyme A:retinol acyltransferase
-
 Homo sapiens
2.3.1.76 ArAT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.76 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.76 7.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.22 additional information
-
 additional information inhibition kinetics depend highly on retinol concentration, overview, recombinant enzyme Homo sapiens
2.3.1.76 0.00045
-
 XP620 ARAT activity of acyl coenzyme A:diacylglycerol acyltransferase isozyme 1, pH 7.4, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.76 0.00011
-
 inhibits the ARAT activity of acyl coenzyme A:diacylglycerol acyltransferase isozyme 1 dependent on the retinol concentration in vivo and in vitro, IC50: 110 nM, no inhibition of isozyme 2 and acyl coenzyme A:monoacylglycerol acyltransferase isozyme 3, ov Homo sapiens XP620