EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.16 | expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.16 | purified recombinant enzyme free or in complex with spermidine, putrescine, 5'-methyl, 5'-deoxymethylthioadenosine, and S-adenosylmethionine, hanging drop vapor diffusion method, 20°C, 10 mg/ml protein solution is mixed with reservoir solution, containing 20-25% PEG 3350, 0.1 M (NH4)2SO4, 0.1 M HEPES-NaOH, pH 7.5, and ligands in a ratio of 1: 5 enzyme-ligand, X-ray diffraction structure determination and analysis at 1.9-2.1 A resolution | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.16 | D101I | site-directed mutagenesis, almost inactive mutant | Thermotoga maritima |
2.5.1.16 | D170A | site-directed mutagenesis, the mutsnt shows reduced activity compared to the wild-type enzyme | Thermotoga maritima |
2.5.1.16 | D173A | site-directed mutagenesis, the mutsnt shows reduced activity compared to the wild-type enzyme | Thermotoga maritima |
2.5.1.16 | Y76F | site-directed mutagenesis, the mutsnt shows reduced activity compared to the wild-type enzyme | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.16 | S-Adenosyl-1,8-diamino-3-thiooctane | - |
Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.16 | additional information | - |
additional information | knetics of wild-type and mutant enzymes | Thermotoga maritima | |
2.5.1.16 | 0.0075 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant enzyme | Thermotoga maritima | |
2.5.1.16 | 0.009 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant enzyme | Homo sapiens | |
2.5.1.16 | 0.019 | - |
putrescine | pH 7.5, 37°C, recombinant enzyme | Thermotoga maritima | |
2.5.1.16 | 0.02 | - |
putrescine | pH 7.5, 37°C, recombinant enzyme | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.16 | S-adenosylmethioninamine + putrescine | Homo sapiens | - |
5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | Thermotoga maritima | - |
5'-S-methyl-5'-thioadenosine + spermidine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.16 | Homo sapiens | P19623 | - |
- |
2.5.1.16 | Thermotoga maritima | Q9WZC2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.16 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.16 | S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | general mechanistic hypothesis for the aminopropyl transfer reaction | Homo sapiens | |
2.5.1.16 | S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | general mechanistic hypothesis for the aminopropyl transfer reaction | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.16 | 5'-methyl,5'-deoxymethylthioadenosine + putrescine | low activity | Homo sapiens | ? | - |
? | |
2.5.1.16 | 5'-methyl,5'-deoxymethylthioadenosine + putrescine | low activity | Thermotoga maritima | ? | - |
? | |
2.5.1.16 | additional information | amine substrate specificity, overview | Homo sapiens | ? | - |
? | |
2.5.1.16 | additional information | amine substrate specificity, overview | Thermotoga maritima | ? | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | - |
Homo sapiens | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | - |
Thermotoga maritima | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | the enzyme is highly specific for putrescine | Homo sapiens | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | the enzyme shows a relatively low substrate specificity for putrescine | Thermotoga maritima | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | low activity | Homo sapiens | spermidine + 5'-methylthioadenosine | - |
? | |
2.5.1.16 | S-adenosylmethioninamine + putrescine | low activity | Thermotoga maritima | spermidine + 5'-methylthioadenosine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.16 | More | structure analysis, comparison to the enzyme from Thermotoga maritima | Homo sapiens |
2.5.1.16 | More | structure analysis, comparison to the human enzyme | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.16 | SPDSYN | - |
Homo sapiens |
2.5.1.16 | SPDSYN | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 37 | - |
assay at | Homo sapiens |
2.5.1.16 | 37 | 80 | assay at | Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 37 | 80 | - |
Thermotoga maritima |
2.5.1.16 | 37 | 50 | - |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 80 | - |
2 min, purified recombinant enzyme, stable | Thermotoga maritima |
2.5.1.16 | 80 | - |
2 min, recombinant enzyme, inactivation | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.16 | additional information | - |
additional information | - |
Thermotoga maritima | |
2.5.1.16 | 0.012 | - |
putrescine | pH 7.5, 80°C, recombinant mutant D101I | Thermotoga maritima | |
2.5.1.16 | 0.012 | - |
S-adenosylmethioninamine | pH 7.5, 80°C, recombinant mutant D101I | Thermotoga maritima | |
2.5.1.16 | 0.014 | - |
putrescine | pH 7.5, 80°C, recombinant mutant D170A | Thermotoga maritima | |
2.5.1.16 | 0.014 | - |
S-adenosylmethioninamine | pH 7.5, 80°C, recombinant mutant D170A | Thermotoga maritima | |
2.5.1.16 | 0.77 | - |
putrescine | pH 7.5, 37°C, recombinant wild-type enzyme | Thermotoga maritima | |
2.5.1.16 | 0.77 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme | Thermotoga maritima | |
2.5.1.16 | 1.5 | - |
putrescine | pH 7.5, 80°C, recombinant mutant D173A | Thermotoga maritima | |
2.5.1.16 | 1.5 | - |
S-adenosylmethioninamine | pH 7.5, 80°C, recombinant mutant D173A | Thermotoga maritima | |
2.5.1.16 | 1.9 | - |
putrescine | pH 7.5, 37°C, recombinant enzyme | Homo sapiens | |
2.5.1.16 | 1.9 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant enzyme | Homo sapiens | |
2.5.1.16 | 8.1 | - |
putrescine | pH 7.5, 80°C, recombinant mutant Y76F | Thermotoga maritima | |
2.5.1.16 | 8.1 | - |
S-adenosylmethioninamine | pH 7.5, 80°C, recombinant mutant Y76F | Thermotoga maritima | |
2.5.1.16 | 22.7 | - |
putrescine | pH 7.5, 80°C, recombinant wild-type enzyme | Thermotoga maritima | |
2.5.1.16 | 22.7 | - |
S-adenosylmethioninamine | pH 7.5, 80°C, recombinant wild-type enzyme | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 7.5 | - |
assay at | Homo sapiens |