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Literature summary extracted from

  • Ruiz-Duenas, F.J.; Morales, M.; Perez-Boada, M.; Choinowski, T.; Martinez, M.J.; Piontek, K.; Martinez, A.T.
    Manganese oxidation site in Pleurotus eryngii versatile peroxidase: a site-directed mutagenesis, kinetic, and crystallographic study (2007), Biochemistry, 46, 66-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.16 expression in Escherichia coli Pleurotus eryngii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.16 hanging drop vapor diffusion method, crystal structures of untreated versatile peroxidase (immediately after expression in Escherichia coli and in vitro reconstitution), native versatile peroxidase (treated with Mn2+), D175A variant, and wild-type verstile peroxidase (from Pleurotus eryngii culture) Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.16 A173R kcat/KM for Mn2+ is 1.4fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.4fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.3fold higher than wild-type value Pleurotus eryngii
1.11.1.16 D175A kcat/KM for Mn2+ is 842fold lower than wild-type value, kcat/Km for veratryl alcohol is3.2 fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.8fold higher than wild-type value Pleurotus eryngii
1.11.1.16 E36A kcat/KM for Mn2+ is 258fold lower than wild-type value, kcat/Km for veratryl alcohol is identical to wild-type value, kcat/Km for Reactive Black 5 is 1.2fold higher than wild-type value Pleurotus eryngii
1.11.1.16 E36A/E40A kcat/KM for Mn2+ is 16000fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.1fold higher than wild-type value Pleurotus eryngii
1.11.1.16 E36A/E40A/D175A kcat for Mn2+ is 149fold lower than wild-type value, kcat/Km for veratryl alcohol is nearly identical to wild-type value, kcat/Km for Reactive Black 5 is 2fold higher than wild-type value Pleurotus eryngii
1.11.1.16 E36A/E40A/D175A/P327ter kcat for Mn2+ is 149fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.6fold lower than wild-type value, kcat/Km for Reactive Black 5 is 2.4fold higher than wild-type value Pleurotus eryngii
1.11.1.16 E36D kcat/KM for Mn2+ is 77fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold higher than wild-type value, kcat/Km for Reactive Black 5 is 3.5fold higher than wild-type value Pleurotus eryngii
1.11.1.16 E40A kcat/KM for Mn2+ is 1231fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.2fold lower than wild-type value, kcat/Km for Reactive Black 5 is nearly identical to wild-type value Pleurotus eryngii
1.11.1.16 E40D kcat/KM for Mn2+ is 54fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold lower than wild-type value, kcat/Km for Reactive Black 5 is 2.4fold higher than wild-type value Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.417
-
 Mn2+ 25°C, pH 5, mutant A173R Pleurotus eryngii
1.11.1.16 4.91
-
 Mn2+ 25°C, pH 5, mutant E40D Pleurotus eryngii
1.11.1.16 4.98
-
 Mn2+ 25°C, pH 5, mutant E36D Pleurotus eryngii
1.11.1.16 11.26
-
 Mn2+ 25°C, pH 5, mutant E40A Pleurotus eryngii
1.11.1.16 13.84
-
 Mn2+ 25°C, pH 5, mutant E36A Pleurotus eryngii
1.11.1.16 16.1
-
 Mn2+ 25°C, pH 5, mutant D175A Pleurotus eryngii
1.11.1.16 46.86
-
 Mn2+ 25°C, pH 5, mutant E36A/E40A Pleurotus eryngii
1.11.1.16 69.97
-
 Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A/P327ter Pleurotus eryngii
1.11.1.16 76.4
-
 Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A Pleurotus eryngii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.16 Ca2+ enzyme contains Ca2+ Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus eryngii O94753 versatile peroxidase VPL2 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.16 recombinant Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 Mn2+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O
-
 ?
1.11.1.16 Reactive Black 5 + H2O2
-
 Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
 ?
1.11.1.16 veratryl alcohol + H2O2 + H+
-
 Pleurotus eryngii veratraldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.16 versatile peroxidase VPL2 precursor
-
 Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 2
-
 Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A Pleurotus eryngii
1.11.1.16 2
-
 Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A/P327ter Pleurotus eryngii
1.11.1.16 5
-
 Mn2+ 25°C, pH 5, mutant E36A/E40A Pleurotus eryngii
1.11.1.16 15
-
 Mn2+ 25°C, pH 5, mutant E40A Pleurotus eryngii
1.11.1.16 32
-
 Mn2+ 25°C, pH 5, mutant D175A Pleurotus eryngii
1.11.1.16 85
-
 Mn2+ 25°C, pH 5, mutant E36A Pleurotus eryngii
1.11.1.16 103
-
 Mn2+ 25°C, pH 5, mutant E36D Pleurotus eryngii
1.11.1.16 145
-
 Mn2+ 25°C, pH 5, mutant E40D Pleurotus eryngii
1.11.1.16 467
-
 Mn2+ 25°C, pH 5, mutant A173R Pleurotus eryngii

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.16 heme enzyme contains heme Pleurotus eryngii