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Literature summary extracted from

  • Sura, G.R.; Lasagna, M.; Gawandi, V.; Reinhart, G.D.; Fitzpatrick, P.F.
    Effects of ligands on the mobility of an active-site loop in tyrosine hydroxylase as monitored by fluorescence anisotropy (2006), Biochemistry, 45, 9632-9638.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expression in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.2 F184W/W372F 4fold lower Km for L-tyrosine compared to the wild-type enzyme. Similar Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
1.14.16.2 W166F/F184W/W233F/W372F mutant protein contains one tryptophan at residue 184 in the middle of a mobile active-site loop. The mutant was generated to perform steady-state fluorescence anisotropy measurements and shows kinetic properties similar to the wild-type enzyme Homo sapiens
1.14.16.2 W166F/F184W/W372F Km for L-tyrosine similar to the wild-type enzyme. 3fold higher Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
1.14.16.2 W166F/W233F/W372F Km for L-tyrosine similar to the wild-type enzyme. 4fold higher Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
1.14.16.2 W166F/W372F Km for L-tyrosine similar to the wild-type enzyme. 3fold higher Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
1.14.16.2 W372F 10fold lower Km for L-tyrosine compared to the wild-type enzyme. Similar Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.045
-
 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine 0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C Homo sapiens
1.14.16.2 0.08
-
 L-tyrosine 0.38 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 Hi-TRAP heparin column Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine + O2
-
 Homo sapiens 3,4-dihydroxyphenylalanine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 TyrH
-
 Homo sapiens
1.14.16.2 tyrosine hydroxylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.16.2 3.03
-
 L-tyrosine 0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C Homo sapiens