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Literature summary extracted from

  • de Carvalho, L.P.; Blanchard, J.S.
    Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis (2006), Biochemistry, 45, 8988-8999.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.13 0.012
-
2-oxo-3-methylbutanoate pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.05
-
crotonyl-CoA hydrolysis, absence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.057
-
acetyl-CoA cosubstrate 2-oxo-butanoate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.074
-
acetyl-CoA cosubstrate pyruvate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.136
-
acetyl-CoA cosubstrate 2-oxo-3-methylbutanoate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.16
-
acetyl-CoA hydrolysis, presence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.22
-
propionyl-CoA hydrolysis, absence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.279
-
acetyl-CoA cosubstrate 2-oxo-n-pentanoate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.37
-
acetyl-CoA hydrolysis, absence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.41
-
2-oxo-n-pentanoate pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.86
-
2-oxo-butanoate pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.95
-
crotonyl-CoA hydrolysis, presence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 9.5
-
pyruvate pH 7.4, 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Mycobacterium tuberculosis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA nonrapid equilibrium bi-bi kinetic mechanism, with a preferred pathway to the ternary complex. Hydrolysis of alpha-isopropylmalyl-CoA is direct, without the formation of a cyclic anhydride intermediate Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Mycobacterium tuberculosis 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2.3.3.13 2-oxo-butanoate + acetyl-CoA + H2O
-
Mycobacterium tuberculosis 2-ethyl-2-hydroxysuccinic acid + CoA
-
?
2.3.3.13 2-oxo-n-pentanoate + acetyl-CoA + H2O
-
Mycobacterium tuberculosis 2-hydroxy-2-propylsuccinic acid + CoA
-
?
2.3.3.13 acetyl-CoA + H2O
-
Mycobacterium tuberculosis acetate + CoA
-
?
2.3.3.13 crotonyl-CoA + H2O
-
Mycobacterium tuberculosis crotonate + CoA
-
?
2.3.3.13 propionyl-CoA + H2O
-
Mycobacterium tuberculosis propanoate + CoA
-
?
2.3.3.13 pyruvate + acetyl-CoA + H2O
-
Mycobacterium tuberculosis 2-hydroxy-2-methylsuccinic acid + CoA
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 0.03
-
acetyl-CoA hydrolysis, presence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.04
-
propionyl-CoA hydrolysis, absence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.04
-
crotonyl-CoA hydrolysis, presence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.05
-
crotonyl-CoA hydrolysis, absence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 0.06
-
acetyl-CoA hydrolysis, absence of MgCl2, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 2.1
-
acetyl-CoA cosubstrate 2-oxo-3-methylbutanoate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 3.5
-
2-oxo-3-methylbutanoate pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 4.5
-
acetyl-CoA cosubstrate pyruvate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 5
-
acetyl-CoA cosubstrate 2-oxo-n-pentanoate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 6.1
-
pyruvate pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 7
-
2-oxo-n-pentanoate pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 7.6
-
acetyl-CoA cosubstrate 2-oxo-butanoate, pH 7.4, 25°C Mycobacterium tuberculosis
2.3.3.13 10.7
-
2-oxo-butanoate pH 7.4, 25°C Mycobacterium tuberculosis