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Literature summary extracted from

  • Williams, L.; Nguyen, T.; Li, Y.; Porter, T.N.; Raushel, F.M.
    Uronate isomerase: a nonhydrolytic member of the amidohydrolase superfamily with an ambivalent requirement for a divalent metal ion (2006), Biochemistry, 45, 7453-7462.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.12 overexpression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.12 D-arabinaric acid competitive inhibitor Escherichia coli
5.3.1.12 D-arabinohydroxamate competitive inhibitor Escherichia coli
5.3.1.12 L-Gulonic acid competitive inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.12 0.3
-
 D-glucuronate reconstituted with Co2+, 30°C, pH 8 Escherichia coli
5.3.1.12 0.48
-
 D-glucuronate reconstituted with Mn2+, 30°C, pH 8 Escherichia coli
5.3.1.12 0.49
-
 D-glucuronate apoenzyme Escherichia coli
5.3.1.12 0.5
-
 D-glucuronate reconstituted with Zn2+, 30°C, pH 8 Escherichia coli
5.3.1.12 0.55
-
 D-glucuronate reconstituted with Cd2+, 30°C, pH 8 Escherichia coli
5.3.1.12 8.5
-
 D-glucuronate reconstituted with Ni2+, 30°C, pH 8 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.12 Zn2+ enzyme contains 1 equiv of zinc per subunit Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.12 Escherichia coli P0A8G3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.12 of the wild type and recombinant protein, ammonium sulfate fractionation, gel filtration, and ion exchange chromatography Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.1.12 D-Glucuronate = D-fructuronate reaction mechanisms in which an active site base abstracts the proton from C2 of D-glucuronate to form a cisenediol intermediate. The conjugate acid then transfers this proton to C1 of the cis-enediol intermediate to form D-fructuronate Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.12 D-Glucuronate
-
 Escherichia coli D-Fructuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.12 Glucuronate isomerase
-
 Escherichia coli
5.3.1.12 Uronate isomerase URI Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.1.12 FAD
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.12 0.000013
-
 D-arabinaric acid apo and Zn2+ reconstituted enzyme Escherichia coli
5.3.1.12 0.00043
-
 L-Gulonic acid Zn2+ reconstituted enzyme Escherichia coli
5.3.1.12 0.00067
-
 D-arabinohydroxamate Zn2+ reconstituted enzyme Escherichia coli
5.3.1.12 0.00093
-
 D-arabinohydroxamate apo enzyme Escherichia coli