EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.3.9 | expression in Escherichia coli | Streptococcus salivarius |
2.7.3.9 | expression in Escherichia coli | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.9 | 12 | - |
protein histidine | at pH 6,1 | Streptococcus salivarius | |
2.7.3.9 | 20 | - |
protein histidine | at pH 7,0 | Streptococcus salivarius | |
2.7.3.9 | 39 | - |
protein histidine | at pH 7,9 | Streptococcus salivarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.3.9 | 130000 | - |
gel filtration | Bacillus subtilis |
2.7.3.9 | 130000 | - |
dimer, gel filtration | Streptococcus salivarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.3.9 | Bacillus subtilis | P08838 | - |
- |
2.7.3.9 | Streptococcus salivarius | P30299 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.3.9 | of the recombinant protein | Streptococcus salivarius |
2.7.3.9 | of the recombinant protein | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.3.9 | 145 | 400 | at 37°C | Streptococcus salivarius |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.3.9 | several months at -80°C, up to 1 h at 37°C | Streptococcus salivarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.9 | phosphoenolpyruvate + protein histidine | HPr, heat stable histidine containing protein, HPr(Ser-P) heat stable histidine containing protein phosphorylated at serine 46, HPr(His-P) heat stable histidine containing protein phosphorylated at histidine 15, HPr-P2 heat stable histidine containing protein doubly phosphorylated, HPr(S46D) heat stable histidine containing protein in which serine is replaced by asparate | Streptococcus salivarius | pyruvate + protein N-pros-phosphohistidine | - |
? | |
2.7.3.9 | phosphoenolpyruvate + protein histidine | HPr, heat stable histidine containing protein, HPr(Ser-P) heat stable histidine containing protein phosphorylated at serine 46, HPr(His-P) heat stable histidine containing protein phosphorylated at histidine 15, HPr-P2 heat stable histidine containing protein doubly phosphorylated, HPr(S46D) heat stable histidine containing protein in which serine is replaced by asparate | Bacillus subtilis | pyruvate + protein N-pros-phosphohistidine | - |
? | |
2.7.3.9 | phosphoenolpyruvate + protein histidine | - |
Streptococcus salivarius | pyruvate + protein Npi-phospho-L-histidine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.3.9 | dimer | - |
Streptococcus salivarius |
2.7.3.9 | dimer | - |
Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.3.9 | enzyme I of the phosphotransferase system | - |
Streptococcus salivarius |
2.7.3.9 | enzyme I of the phosphotransferase system | - |
Bacillus subtilis |
2.7.3.9 | PEP:sugar phosphotransferase system enzyme I | - |
Streptococcus salivarius |
2.7.3.9 | PEP:sugar phosphotransferase system enzyme I | - |
Bacillus subtilis |
2.7.3.9 | phosphoenolpyruvate-protein phosphotransferase | - |
Streptococcus salivarius |
2.7.3.9 | phosphoenolpyruvate-protein phosphotransferase | - |
Bacillus subtilis |
2.7.3.9 | phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Streptococcus salivarius |
2.7.3.9 | phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Bacillus subtilis |
2.7.3.9 | phosphotransferase system enzyme I | - |
Streptococcus salivarius |
2.7.3.9 | phosphotransferase system enzyme I | - |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.3.9 | 5.9 | - |
HPr(Ser-P) as substrate | Streptococcus salivarius |
2.7.3.9 | 6.1 | - |
HPr(S46D) as substrate | Streptococcus salivarius |
2.7.3.9 | 6.1 | - |
HPr(Ser-P) as substrate | Bacillus subtilis |
2.7.3.9 | 7 | - |
HPr as substrate | Streptococcus salivarius |
2.7.3.9 | 7.9 | - |
HPr as substrate | Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.3.9 | 5 | 7.5 | HPr(Ser-P) and HPr(S46D) as substrates | Streptococcus salivarius |
2.7.3.9 | 5.9 | 8.5 | HPr as substrate | Streptococcus salivarius |
2.7.3.9 | 5.9 | 7.5 | HPr(Ser-P) as substrate | Bacillus subtilis |
2.7.3.9 | 6.5 | 8.5 | HPr as substrate | Bacillus subtilis |