BRENDA - Enzyme Database

Synergistic, random sequential binding of substrates in cobalamin-independent methionine synthase

Taurog, R.E.; Jakubowski, H.; Matthews, R.G.; Biochemistry 45, 5083-5091 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.14
expressed in Escherichia coli strain K-12
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Escherichia coli
-
-
-
2.1.1.14
Escherichia coli pJG816
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.14
DEAE column chromatography
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
672156
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
672156
Escherichia coli pJG816
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.12
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
at 25C
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
vitamin B12 independent enzyme
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
expressed in Escherichia coli strain K-12
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
vitamin B12 independent enzyme
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
DEAE column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
672156
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
672156
Escherichia coli pJG816
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.12
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
at 25C
Escherichia coli