EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.6 | expressed in Escherichia coli BL21(DE3) cells | Borreliella burgdorferi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.6 | E43I | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | E43Q | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | G29A | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | H242A | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | H242L | shows almost no change in the catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | H242W | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | T31G | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | T31S | shows almost no change in the catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | W220A | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | W220L | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | W220Y | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | Y269F | 6fold drop in the catalytic efficiency compared to the wild type enzyme | Borreliella burgdorferi |
6.1.1.6 | Y269S | significant decrease of catalytic activity compared to the wild type enzyme | Borreliella burgdorferi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | S-(2-aminoethyl)-L-cysteine | competitive inhibitor | Borreliella burgdorferi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.18 | - |
L-lysine | mutant enzyme H242L | Borreliella burgdorferi | |
6.1.1.6 | 0.23 | - |
L-lysine | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 0.33 | - |
L-lysine | mutant enzyme W220A | Borreliella burgdorferi | |
6.1.1.6 | 0.4 | - |
L-lysine | mutant enzyme Y269F | Borreliella burgdorferi | |
6.1.1.6 | 0.76 | - |
L-lysine | mutant enzyme T31S | Borreliella burgdorferi | |
6.1.1.6 | 0.83 | - |
tRNALysUUU | mutant enzyme Y269F | Borreliella burgdorferi | |
6.1.1.6 | 1 | - |
tRNALysUUU | mutant enzyme H242A | Borreliella burgdorferi | |
6.1.1.6 | 1.1 | - |
tRNALysUUU | mutant enzyme W220L | Borreliella burgdorferi | |
6.1.1.6 | 1.1 | - |
tRNALysUUU | mutant enzyme Y269S | Borreliella burgdorferi | |
6.1.1.6 | 1.2 | - |
tRNALysUUU | mutant enzyme W220Y | Borreliella burgdorferi | |
6.1.1.6 | 1.3 | - |
ATP | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 1.3 | - |
tRNALysUUU | mutant enzyme H242L | Borreliella burgdorferi | |
6.1.1.6 | 1.6 | - |
ATP | mutant enzyme Y269F | Borreliella burgdorferi | |
6.1.1.6 | 1.7 | - |
ATP | mutant enzyme T31S | Borreliella burgdorferi | |
6.1.1.6 | 2 | - |
tRNALysUUU | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 2.1 | - |
tRNALysUUU | mutant enzyme T31G | Borreliella burgdorferi | |
6.1.1.6 | 2.1 | - |
tRNALysUUU | mutant enzyme T31S | Borreliella burgdorferi | |
6.1.1.6 | 2.5 | - |
tRNALysUUU | mutant enzyme W220A | Borreliella burgdorferi | |
6.1.1.6 | 2.8 | - |
L-lysine | mutant enzyme Y269S | Borreliella burgdorferi | |
6.1.1.6 | 3.3 | - |
L-lysine | mutant enzyme H242A | Borreliella burgdorferi | |
6.1.1.6 | 3.3 | - |
L-lysine | mutant enzyme W220L | Borreliella burgdorferi | |
6.1.1.6 | 4.4 | - |
L-lysine | mutant enzyme T31G | Borreliella burgdorferi | |
6.1.1.6 | 4.7 | - |
L-lysine | mutant enzyme W220Y | Borreliella burgdorferi | |
6.1.1.6 | 5.1 | - |
tRNALysUUU | mutant enzyme G29A | Borreliella burgdorferi | |
6.1.1.6 | 6.3 | - |
L-lysine | mutant enzyme G29A | Borreliella burgdorferi | |
6.1.1.6 | 6.9 | - |
ATP | mutant enzyme Y269S | Borreliella burgdorferi | |
6.1.1.6 | 9.5 | - |
ATP | mutant enzyme T31G | Borreliella burgdorferi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.6 | Borreliella burgdorferi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.6 | nickel affinity column chromatography Superose 6 column chromatography | Borreliella burgdorferi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.6 | ATP + L-lysine + tRNALysUUU | - |
Borreliella burgdorferi | AMP + L-lysyl-tRNALysUUU + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.6 | class I lysyl-tRNA synthetase | - |
Borreliella burgdorferi |
6.1.1.6 | LysRS1 | - |
Borreliella burgdorferi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.00012 | - |
L-lysine | mutant enzyme H242A | Borreliella burgdorferi | |
6.1.1.6 | 0.00012 | - |
tRNALysUUU | mutant enzyme W220L | Borreliella burgdorferi | |
6.1.1.6 | 0.00013 | - |
tRNALysUUU | mutant enzyme H242A | Borreliella burgdorferi | |
6.1.1.6 | 0.00017 | - |
L-lysine | mutant enzyme W220L | Borreliella burgdorferi | |
6.1.1.6 | 0.00051 | - |
ATP | mutant enzyme Y269S | Borreliella burgdorferi | |
6.1.1.6 | 0.00056 | - |
tRNALysUUU | mutant enzyme W220A | Borreliella burgdorferi | |
6.1.1.6 | 0.00075 | - |
L-lysine | mutant enzyme W220A | Borreliella burgdorferi | |
6.1.1.6 | 0.0011 | - |
ATP | mutant enzyme T31G | Borreliella burgdorferi | |
6.1.1.6 | 0.0023 | - |
tRNALysUUU | mutant enzyme Y269S | Borreliella burgdorferi | |
6.1.1.6 | 0.0026 | - |
L-lysine | mutant enzyme Y269S | Borreliella burgdorferi | |
6.1.1.6 | 0.0046 | - |
L-lysine | mutant enzyme T31G | Borreliella burgdorferi | |
6.1.1.6 | 0.005 | - |
tRNALysUUU | mutant enzyme T31G | Borreliella burgdorferi | |
6.1.1.6 | 0.017 | - |
ATP | mutant enzyme Y269F | Borreliella burgdorferi | |
6.1.1.6 | 0.099 | - |
L-lysine | mutant enzyme Y269F | Borreliella burgdorferi | |
6.1.1.6 | 0.13 | - |
L-lysine | mutant enzyme W220Y | Borreliella burgdorferi | |
6.1.1.6 | 0.14 | - |
tRNALysUUU | mutant enzyme Y269F | Borreliella burgdorferi | |
6.1.1.6 | 0.23 | - |
tRNALysUUU | mutant enzyme W220Y | Borreliella burgdorferi | |
6.1.1.6 | 0.29 | - |
L-lysine | mutant enzyme H242L | Borreliella burgdorferi | |
6.1.1.6 | 0.29 | - |
tRNALysUUU | mutant enzyme H242L | Borreliella burgdorferi | |
6.1.1.6 | 0.33 | - |
tRNALysUUU | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 0.34 | - |
L-lysine | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 0.45 | - |
L-lysine | mutant enzyme G29A | Borreliella burgdorferi | |
6.1.1.6 | 0.53 | - |
L-lysine | mutant enzyme T31S | Borreliella burgdorferi | |
6.1.1.6 | 0.57 | - |
ATP | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 0.59 | - |
tRNALysUUU | mutant enzyme G29A | Borreliella burgdorferi | |
6.1.1.6 | 0.68 | - |
tRNALysUUU | mutant enzyme T31S | Borreliella burgdorferi | |
6.1.1.6 | 1.7 | - |
ATP | mutant enzyme T31S | Borreliella burgdorferi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | ATP | - |
Borreliella burgdorferi |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 2.9 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme H242L | Borreliella burgdorferi | |
6.1.1.6 | 3.6 | - |
S-(2-aminoethyl)-L-cysteine | wild type enzyme | Borreliella burgdorferi | |
6.1.1.6 | 8.5 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme H242A | Borreliella burgdorferi | |
6.1.1.6 | 11 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme W220L | Borreliella burgdorferi | |
6.1.1.6 | 11.8 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme G29A | Borreliella burgdorferi | |
6.1.1.6 | 17 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme W220Y | Borreliella burgdorferi | |
6.1.1.6 | 19 | - |
S-(2-aminoethyl)-L-cysteine | mutant enzyme W220A | Borreliella burgdorferi |