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Literature summary extracted from

  • Guo, H.; Li, L.; Wang, P.G.
    Biochemical characterization of UDP-GlcNAc/Glc 4-epimerase from Escherichia coli O86:B7 (2006), Biochemistry, 45, 13760-13768.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.2 S306Y plasmid containing the Gne S306Y constructed using the QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA).The S306Y mutation totally abolished activity toward the acetylated substrate. Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.2 0.295
-
 UDP-Gal
-
 Escherichia coli
5.1.3.2 0.323
-
 UDP-GlcNAc
-
 Escherichia coli
5.1.3.2 0.37
-
 UDP-Glc
-
 Escherichia coli
5.1.3.2 0.373
-
 UDP-GalNAc
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 38800
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.2 UDP-Glc Escherichia coli
-
 UDP-Gal
-
 r
5.1.3.2 UDP-Glc Escherichia coli O86:B7
-
 UDP-Gal
-
 r
5.1.3.2 UDP-GlcNAc Escherichia coli
-
 UDP-GalNAc
-
 r
5.1.3.2 UDP-GlcNAc Escherichia coli O86:B7
-
 UDP-GalNAc
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Escherichia coli
-
 O86:B7
-
5.1.3.2 Escherichia coli O86:B7
-
 O86:B7
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-Glc
-
 Escherichia coli UDP-Gal
-
 r
5.1.3.2 UDP-Glc
-
 Escherichia coli O86:B7 UDP-Gal
-
 r
5.1.3.2 UDP-GlcNAc
-
 Escherichia coli UDP-GalNAc
-
 r
5.1.3.2 UDP-GlcNAc
-
 Escherichia coli O86:B7 UDP-GalNAc
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.3.2 dimer 2 * 38800, gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 GNE
-
 Escherichia coli
5.1.3.2 UDP-GlcNAc/Glc 4-epimerase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.2 37
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.2 10 40
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.2 7 9
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.2 Escherichia coli
-
-
 6.8