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Literature summary extracted from

  • Muniz, A.; Villazana-Espinoza, E.T.; Thackeray, B.; Tsin, A.T.
    11-cis-Acyl-CoA:retinol O-acyltransferase activity in the primary culture of chicken Muller cells (2006), Biochemistry, 45, 12265-12273.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.76 additional information in presence of palmitoyl-CoA and CRALBP, Muller cell membranes synthesize 11-cis-retinyl ester from 11-cis-retinol at a rate which is 20-fold higher than that of all-trans-retinyl ester, in absence of CRALBP, 11-cis-retinyl ester synthesis is greatly reduced by 7-fold, in absence of palmitoyl-CoA, retinyl ester synthesis is not observed Gallus gallus
2.3.1.135 cellular retinol-binding protein i.e. CRALBP, in presence of palmitoyl-CoA and CRALBP, Muller cell membranes synthesize 11-cis-retinyl ester from 11-cis-retinol at a rate which is 20fold higher than that of all-trans-retinyl ester, in absence of CRALBP, 11-cis-retinyl ester synthesis is greatly reduced by 7fold Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.76 progesterone
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.76 additional information
-
 additional information kinetics Gallus gallus
2.3.1.135 additional information
-
 additional information kinetics Gallus gallus
2.3.1.135 0.0113
-
 11-cis-retinol-[cellular retinol-binding protein] pH 8.0, 22°C Gallus gallus
2.3.1.135 0.0288
-
 all-trans-retinol-[cellular retinol-binding protein] pH 8.0, 22°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.76 membrane
-
 Gallus gallus 16020
-
2.3.1.135 membrane
-
 Gallus gallus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.76 palmitoyl-CoA + 11-cis-retinol Gallus gallus esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells CoA + 11-cis-retinyl palmitate
-
 ?
2.3.1.76 palmitoyl-CoA + all-trans-retinol Gallus gallus esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells CoA + all-trans-retinyl palmitate
-
 ?
2.3.1.135 palmitoyl-CoA + 11-cis-retinol-[cellular retinol-binding protein] Gallus gallus esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells CoA + 11-cis-retinyl palmitate-[cellular retinol-binding protein]
-
 ?
2.3.1.135 palmitoyl-CoA + all-trans-retinol-[cellular retinol-binding protein] Gallus gallus esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells CoA + all-trans-retinyl palmitate-[cellular retinol-binding protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.76 Gallus gallus
-
-
-
2.3.1.135 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.76 native enzyme partially by membrane preparation Gallus gallus
2.3.1.135 native enzyme partially by membrane preparation Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.76 Muller cell primary cell culture Gallus gallus
-
2.3.1.76 retina
-
 Gallus gallus
-
2.3.1.135 Muller cell primary cell culture Gallus gallus
-
2.3.1.135 retina
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.76 additional information
-
-
 Gallus gallus
2.3.1.76 0.000023
-
 11-cis-retinyl ester synthesis in absence of cellular retinol-binding protein Gallus gallus
2.3.1.135 additional information
-
-
 Gallus gallus
2.3.1.135 0.166
-
 11-cis-retinyl ester synthesis in presence of cellular retinol-binding protein Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.76 palmitoyl-CoA + 11-cis-retinol
-
 Gallus gallus CoA + 11-cis-retinyl palmitate
-
 ?
2.3.1.76 palmitoyl-CoA + 11-cis-retinol esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells Gallus gallus CoA + 11-cis-retinyl palmitate
-
 ?
2.3.1.76 palmitoyl-CoA + all-trans-retinol
-
 Gallus gallus CoA + all-trans-retinyl palmitate
-
 ?
2.3.1.76 palmitoyl-CoA + all-trans-retinol esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells Gallus gallus CoA + all-trans-retinyl palmitate
-
 ?
2.3.1.135 additional information in presence of palmitoyl-CoA and CRALBP, Muller cell membranes synthesize 11-cis-retinyl ester from 11-cis-retinol at a rate which is 20fold higher than that of all-trans-retinyl ester, in absence of CRALBP, 11-cis-retinyl ester synthesis is greatly reduced by 7fold, in absence of palmitoyl-CoA, retinyl ester synthesis is not observed Gallus gallus ?
-
 ?
2.3.1.135 palmitoyl-CoA + 11-cis-retinol-[cellular retinol-binding protein]
-
 Gallus gallus CoA + 11-cis-retinyl palmitate-[cellular retinol-binding protein]
-
 ?
2.3.1.135 palmitoyl-CoA + 11-cis-retinol-[cellular retinol-binding protein] esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells Gallus gallus CoA + 11-cis-retinyl palmitate-[cellular retinol-binding protein]
-
 ?
2.3.1.135 palmitoyl-CoA + all-trans-retinol-[cellular retinol-binding protein]
-
 Gallus gallus CoA + all-trans-retinyl palmitate-[cellular retinol-binding protein]
-
 ?
2.3.1.135 palmitoyl-CoA + all-trans-retinol-[cellular retinol-binding protein] esterification of 11-cis-retinol is four times greater than esterification of all-trans-retinol in Muller cells Gallus gallus CoA + all-trans-retinyl palmitate-[cellular retinol-binding protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.76 11-cis-acyl-CoA:retinol O-acyltransferase
-
 Gallus gallus
2.3.1.76 11-cis-ARAT
-
 Gallus gallus
2.3.1.76 More cf. EC 2.3.1.135 Gallus gallus
2.3.1.135 11-cis-acyl-CoA:retinol O-acyltransferase
-
 Gallus gallus
2.3.1.135 More cf. EC 2.3.1.76 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.76 22
-
 asay at room temperature Gallus gallus
2.3.1.135 22
-
 assay at room temperature Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.76 8
-
 assay at Gallus gallus
2.3.1.135 8
-
 assay at Gallus gallus