Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Qian, J.; West, A.H.; Cook, P.F.
    Acid-base chemical mechanism of homocitrate synthase from Saccharomyces cerevisiae (2006), Biochemistry, 45, 12136-12143.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.14 2-oxoglutarate
-
 Saccharomyces cerevisiae
2.3.3.14 2-Oxomalonate
-
 Saccharomyces cerevisiae
2.3.3.14 Glutarate
-
 Saccharomyces cerevisiae
2.3.3.14 glyoxylate competitive, analysis of pH-dependence of inhibition Saccharomyces cerevisiae
2.3.3.14 oxalate
-
 Saccharomyces cerevisiae
2.3.3.14 oxaloacetate
-
 Saccharomyces cerevisiae
2.3.3.14 oxalylglycine
-
 Saccharomyces cerevisiae
2.3.3.14 pyridine-2,3-dicarboxylate
-
 Saccharomyces cerevisiae
2.3.3.14 Pyridine-2,4-dicarboxylate
-
 Saccharomyces cerevisiae
2.3.3.14 Pyridine-2,5-dicarboxylate
-
 Saccharomyces cerevisiae
2.3.3.14 pyridine-2,6-dicarboxylate
-
 Saccharomyces cerevisiae
2.3.3.14 pyridine-2-carboxylate
-
 Saccharomyces cerevisiae
2.3.3.14 succinyl phosphonate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.14 Zinc bound in the active site Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Saccharomyces cerevisiae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate = (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA mechanism: 2-oxoglutarate first binds to the active site zinc via its alpha-carboxylate and 2-oxo groups, followed by acetyl-CoA. A general base then accepts a proton from the methyl of acetyl-CoA, and a general acid protonates the carbonyl of 2-oxoglutarate in the formation of homocitryl-CoA. The general acid then acts as a base in deprotonating Zn-OH2 in the hydrolysis of homocitryl-CoA to give homocitrate and CoA Saccharomyces cerevisiae
a

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.14 0.4
-
 glyoxylate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 0.43
-
 pyridine-2-carboxylate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 0.6
-
 Pyridine-2,4-dicarboxylate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 0.7
-
 pyridine-2,6-dicarboxylate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 1
-
 oxalate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 3
-
 pyridine-2,3-dicarboxylate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 4
-
 oxaloacetate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 7.7
-
 oxalylglycine pH 7.2 Saccharomyces cerevisiae
2.3.3.14 10
-
 2-Oxomalonate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 14
-
 Pyridine-2,5-dicarboxylate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 26
-
 2-oxoglutarate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 26
-
 Glutarate pH 7.2 Saccharomyces cerevisiae
2.3.3.14 46
-
 succinyl phosphonate pH 7.2 Saccharomyces cerevisiae