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Literature summary extracted from

  • O'Sullivan, S.M.; McCarthy, R.M.; Vargo, M.A.; Colman, R.F.; Sheehan, D.
    Chemical modification at subunit 1 of rat kidney alpha class glutathione transferase with 2,3,5,6-tetrachloro-1,4-benzoquinone: close structural connectivity between glutathione conjugation activity and non-substrate ligand binding (2006), Biochem. Pharmacol., 71, 1629-1636.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 2,3,5,6-tetrachloro-1,4-benzoquinone i.e. TCBQ, inhibition of GSTA1-2 and GSTA1-1 by 70-80%, and GSTA2-2 by 25%, binds to cysteine residues of the enzyme, e.g. Cys17 of GSTA1-1, via a first reversible step to covalent binding, overview, Kitz-Wilson inactivation model Rattus norvegicus
2.5.1.18 dinitrophenyl-glutathione
-
 Rattus norvegicus
2.5.1.18 S-hexyl-glutathione
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol alpha class glutathione transferase Rattus norvegicus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Rattus norvegicus
-
 alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 native alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1 from kidney by anion exchange and glutathione affinity chromatography, followed by another step of ion exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 kidney mainly alpha class glutathione transferase Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Rattus norvegicus chloride + 2,4-dinitrophenyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 More homology modeling of recombinant isozyme GSTA1-1 with and without bound inhibitor 2,3,5,6-tetrachloro-1,4-benzoquinone Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 alpha class glutathione transferase
-
 Rattus norvegicus
2.5.1.18 GST
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7
-
 assay at Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.18 additional information
-
 additional information inhibition kinetics Rattus norvegicus