EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.57 | expressed in Escherichia coli | Homo sapiens |
2.5.1.132 | expressed in Escherichia coli BL21(DE3) Gold cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.57 | M42L | mutant shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity like the wild type enzyme | Homo sapiens |
2.5.1.57 | M42T | the mutation abolishes the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity completely without compromising the Neu5Ac-9-P synthase activity | Homo sapiens |
2.5.1.132 | M42L | the M42L mutant of N-acetylneuraminic acid-9-phosphate synthase, like the wild type enzyme, shows the additional 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
2.5.1.132 | M42T | the mutation of N-acetylneuraminic acid-9-phosphate synthase abolishes its 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
2.5.1.132 | R96K/R100S/E103Q/V105I/V195A | the mutant of N-acetylneuraminic acid-9-phosphate synthase has significant 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.57 | 1.04 | - |
N-acetyl-D-mannosamine 6-phosphate | wild type enzyme, at 37°C | Homo sapiens | |
2.5.1.57 | 1.26 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42T, at 37°C | Homo sapiens | |
2.5.1.57 | 1.72 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
2.5.1.57 | 2.5 | 5 | D-mannose 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
2.5.1.57 | 2.62 | - |
D-mannose 6-phosphate | wild type enzyme, at 37°C | Homo sapiens | |
2.5.1.132 | 2.5 | 5 | D-mannose 6-phosphate | mutant enzyme M42L, at pH 7.5 and 37°C | Homo sapiens | |
2.5.1.132 | 2.62 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.57 | Mg2+ | required | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.132 | phosphoenolpyruvate + D-mannose 6-phosphate + H2O | Homo sapiens | - |
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.57 | Homo sapiens | - |
- |
- |
2.5.1.132 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.57 | Ni2+ chelating Sepharose column chromatography | Homo sapiens |
2.5.1.132 | nickel affinity column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.57 | D-mannose 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate | - |
? | |
2.5.1.57 | N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? | |
2.5.1.132 | phosphoenolpyruvate + D-mannose 6-phosphate + H2O | - |
Homo sapiens | 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.57 | N-acetylneuraminic acid 9-phosphate synthase | - |
Homo sapiens |
2.5.1.57 | Neu5Ac 9-phosphate synthase | - |
Homo sapiens |
2.5.1.132 | 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase | - |
Homo sapiens |
2.5.1.132 | Neu5Ac 9-phosphate synthase | shows also -keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
2.5.1.132 | neu5ac-9-P synthase | shows also -keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthase activity | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.57 | 0.008 | - |
D-mannose 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
2.5.1.57 | 0.011 | - |
D-mannose 6-phosphate | wild type enzyme, at 37°C | Homo sapiens | |
2.5.1.57 | 0.013 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42T, at 37°C | Homo sapiens | |
2.5.1.57 | 0.016 | - |
N-acetyl-D-mannosamine 6-phosphate | mutant enzyme M42L, at 37°C | Homo sapiens | |
2.5.1.57 | 0.022 | - |
N-acetyl-D-mannosamine 6-phosphate | wild type enzyme, at 37°C | Homo sapiens | |
2.5.1.132 | 0.0011 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
2.5.1.132 | 0.0083 | - |
D-mannose 6-phosphate | mutant enzyme M42L, at pH 7.5 and 37°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.132 | 0.000327 | - |
D-mannose 6-phosphate | mutant enzyme M42L, at pH 7.5 and 37°C | Homo sapiens | |
2.5.1.132 | 0.000426 | - |
D-mannose 6-phosphate | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |