Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Awama, A.M.; Mazon, H.; Vial, C.; Marcillat, O.
    Despite its high similarity with monomeric arginine kinase, muscle creatine kinase is only enzymatically active as a dimer (2007), Arch. Biochem. Biophys., 458, 158-166.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 W210Y mutation in the interface of enzyme dimer, dissociates more readily than wild-type to monomer. Dissociation equilibrium constant is 9.7 nM compared to 0.017 nM for wild-type Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.3.2 when incubated with 0.8 M guanidine hydrochloride, MM-CK accumulates as a monomeric molten globule which totally lost its enzymatic activity Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.2 guanidine hydrochloride in the absence of added guanidine hydrochloride, MM-CK activity slightly decreases with NaCl concentration up to 4 M, but a dramatic decline is observed above that value, with full inactivation at 4.5 M. When guanidine is added, curves with similar shapes are obtained but NaCl concentrations needed to inactivate the enzyme are shifted towards lower values Oryctolagus cuniculus
2.7.3.2 Guanidinium chloride inhibitory, in presence of NaCl, increased inhibitory activity. Inactivation by NaCl is due to dissociation of dimeric creatine kinase into its constitutive subunits, and upon monomerization, the protein becomes more susceptible to guanidinium denaturing effect Oryctolagus cuniculus
2.7.3.2 NaCl enzyme activity slightly decreases with NaCl concentration up to 4 M, and a dramatic decline is observed above that value, with full inactivation at 4.5 M. In presence of guanidinium chloride, inactivation occurs much earlier. Inactivation by NaCl is due to dissociation of dimeric creatine kinase into its constitutive subunits, and upon monomerization, the protein becomes more susceptible to guanidinium denaturing effect; in the absence of added guanidine hydrochloride, MM-CK activity slightly decreases with NaCl concentration up to 4 M, but a dramatic decline is observed above that value, with full inactivation at 4.5 M. When guanidine is added, curves with similar shapes are obtained but NaCl concentrations needed to inactivate the enzyme are shifted towards lower values Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Oryctolagus cuniculus P00563
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Oryctolagus cuniculus ADP + phosphocreatine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.2 dimer muscle creatine kinase is only enzymatically active as a dimer Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 MM-CK
-
 Oryctolagus cuniculus