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Literature summary extracted from

  • Nes, W.D.; Sinha, A.; Jayasimha, P.; Zhou, W.; Song, Z.; Dennis, A.L.
    Probing the sterol binding site of soybean sterol methyltransferase by site-directed mutagenesis: functional analysis of conserved aromatic amino acids in Region 1 (2006), Arch. Biochem. Biophys., 448, 23-30.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.142 degradation the amino acids of Region 1 provide a tight substrate orientation imposed by hydrophobic interactions between the sterol side chain and the SMT active site contacts and control the production and processing of the transmethylation pathways governed by the first and second C1-transfer activities Glycine max

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.142 overexpression of the pET vector in Escherichia coli BL21(DE3) Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.142 F82I continues to catalyze strongly both the first and second C1-transfer activities and generates product sets similar to the control Glycine max
2.1.1.142 F82L first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity Glycine max
2.1.1.142 F85L first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity Glycine max
2.1.1.142 F91L first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity Glycine max
2.1.1.142 F93L first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity Glycine max
2.1.1.142 W87L first and second C1-transfer activities are greatly reduced from the control activities Glycine max
2.1.1.142 Y83F first C1-transfer activity is relatively unaffected whereas the second C1-transfer activity generates different amounts of product compared to the control Glycine max
2.1.1.142 Y83F Km and kcat, of wild-type enzyme and mutant enzyme are similar for cycloartenol as substrate Glycine max
2.1.1.142 Y83L first C1-transfer activity greatly reduced from the control activity, loss of the second C1-transfer activity Glycine max
2.1.1.142 Y83L Km and kcat, of wild-type enzyme and mutant enzyme are similar for cycloartenol as substrate Glycine max
2.1.1.143 Y83F Km-value for 24-methylenelophenol is identical to wild-type value, turnover number is 5fold higher than wild-type value Glycine max
2.1.1.143 Y83L inactive mutant enzyme Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.142 0.045
-
 Cycloartenol
-
 Glycine max
2.1.1.142 0.045
-
 Cycloartenol wild-type and mutants Y83F and Y83L Glycine max
2.1.1.142 0.05
-
 24(28)-methylene lophenol wild-type Glycine max
2.1.1.142 0.05
-
 24(28)-methylene lophenol mutant Y83F Glycine max
2.1.1.143 0.05
-
 24-methylenelophenol wild-type enzyme and mutant enzyme Y83F Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.142 160000
-
 wild-type and mutants Y83F and Y83L, gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Glycine max
-
-
-
2.1.1.143 Glycine max
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 24(28)-methylene lophenol + S-adenosyl-L-methionine
-
 Glycine max ?
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine
-
 Glycine max 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 S-adenosyl-L-methionine + cycloartenol
-
 Glycine max S-adenosyl-L-homocysteine + (24R)-24-methylcycloart-25-en-3beta-ol
-
 ?
2.1.1.143 S-adenosyl-L-methionine + 24-methylenelophenol
-
 Glycine max S-adenosyl-L-homocysteine + (Z)-24-ethylidenelophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.142 SMT
-
 Glycine max
2.1.1.142 sterol methyltransferase
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.142 0.001
-
 24(28)-methylene lophenol wild-type Glycine max
2.1.1.142 0.005
-
 24(28)-methylene lophenol mutant Y83F Glycine max
2.1.1.142 0.01
-
 Cycloartenol
-
 Glycine max
2.1.1.142 0.01
-
 Cycloartenol wild-type and mutants Y83F and Y83L Glycine max
2.1.1.143 0.001
-
 24-methylenelophenol wild-type enzyme Glycine max
2.1.1.143 0.005
-
 24-methylenelophenol mutant enzyme Y83F Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.142 5.8 8.9
-
 Glycine max
2.1.1.143 6.2 7.7
-
 Glycine max