EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | dithiothreitol | Dithiothreitol at a concentration of 0.2 mM stimulates enzyme activity by 158% | Acetivibrio thermocellus | |
2.7.7.43 | DTT | maximum activity in the presence of 0.2 mM DTT | Acetivibrio thermocellus | |
2.7.7.43 | additional information | mercaptoethanol has no stimulatory effect | Acetivibrio thermocellus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.43 | cloned in Escherichia coli XL-10 | Acetivibrio thermocellus |
2.7.7.43 | expressed in Escherichia coli strain BL21 | Acetivibrio thermocellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | 4-(hydroxymercuri) benzoic acid | - |
Acetivibrio thermocellus | |
2.7.7.43 | 4-(hydroxymercuri)benzoic acid | - |
Acetivibrio thermocellus | |
2.7.7.43 | Ca2+ | 95% inhibition of enzyme activity at 10 mM | Acetivibrio thermocellus | |
2.7.7.43 | Cu2+ | 97% inhibition of enzyme activity at 10 mM | Acetivibrio thermocellus | |
2.7.7.43 | Fe3+ | 91% inhibition of enzyme activity at 10 mM | Acetivibrio thermocellus | |
2.7.7.43 | additional information | the addition of monovalent cations (K+, Na+) to the reaction mixtures has no effect on the enzyme activity | Acetivibrio thermocellus | |
2.7.7.43 | N-ethylmaleimide | - |
Acetivibrio thermocellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 0.13 | - |
NeuAc | - |
Acetivibrio thermocellus | |
2.7.7.43 | 0.13 | - |
N-acylneuraminate | at 50°C, 10 mM MnCl2, 50 mM Tris buffer (pH 9.5) | Acetivibrio thermocellus | |
2.7.7.43 | 0.16 | - |
NeuGc | - |
Acetivibrio thermocellus | |
2.7.7.43 | 0.16 | - |
N-glycolylneuraminic acid | at 50°C, 10 mM MnCl2, 50 mM Tris buffer (pH 9.5) | Acetivibrio thermocellus | |
2.7.7.43 | 0.24 | - |
CTP | - |
Acetivibrio thermocellus | |
2.7.7.43 | 0.24 | - |
CTP | at 50°C, 10 mM MnCl2, 50 mM Tris buffer (pH 9.5) | Acetivibrio thermocellus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | Co2+ | 66% as effective as Mn2+ | Acetivibrio thermocellus | |
2.7.7.43 | Co2+ | can replace Mn2+, 66% as effective as Mn2+ at 10 mM | Acetivibrio thermocellus | |
2.7.7.43 | Mg2+ | 20 mM | Acetivibrio thermocellus | |
2.7.7.43 | Mg2+ | 57% as effective as Mn2+ | Acetivibrio thermocellus | |
2.7.7.43 | Mg2+ | can replace Mn2+, 57% as effective as Mn2+ at 10 mM | Acetivibrio thermocellus | |
2.7.7.43 | Mn2+ | absolute requirement for a divalent cation for activity, maximum activity at 10 mM Mn2+ | Acetivibrio thermocellus | |
2.7.7.43 | Mn2+ | the enzyme has an absolute requirement for a divalent cation for activity and reaches maximum activity in the presence of 10 mM Mn2+ | Acetivibrio thermocellus | |
2.7.7.43 | Zn2+ | 62% as effective as Mn2+ | Acetivibrio thermocellus | |
2.7.7.43 | Zn2+ | can replace Mn2+, 62% as effective as Mn2+ at 10 mM | Acetivibrio thermocellus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 26000 | - |
SDS-PAGE | Acetivibrio thermocellus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.43 | Acetivibrio thermocellus | - |
strain ATCC 27405 | - |
2.7.7.43 | Acetivibrio thermocellus | - |
ATCC27405 | - |
2.7.7.43 | Acetivibrio thermocellus ATCC 27405 | - |
ATCC27405 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.43 | Ni-NTA column chromatography | Acetivibrio thermocellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.43 | CTP + N-acylneuraminate | - |
Acetivibrio thermocellus | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Acetivibrio thermocellus ATCC 27405 | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-glycolylneuraminic acid | - |
Acetivibrio thermocellus | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | CTP + N-glycolylneuraminic acid | - |
Acetivibrio thermocellus ATCC 27405 | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | N-acetylneuraminic acid + CTP | bi-bi catalytic mechanism, enzyme also accepts N-glycolylneuraminic acid (NeuGc) as a substrate | Acetivibrio thermocellus | CMP-NeuAc + diphosphate | - |
? | |
2.7.7.43 | N-acetylneuraminic acid + CTP | bi-bi catalytic mechanism, enzyme also accepts N-glycolylneuraminic acid (NeuGc) as a substrate | Acetivibrio thermocellus ATCC 27405 | CMP-NeuAc + diphosphate | - |
? | |
2.7.7.43 | N-glycolylneuraminic acid + CTP | - |
Acetivibrio thermocellus | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | N-glycolylneuraminic acid + CTP | - |
Acetivibrio thermocellus ATCC 27405 | CMP-N-glycolylneuraminate + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.43 | CMP-N-acylneuraminic acid synthetase | - |
Acetivibrio thermocellus |
2.7.7.43 | CMP-NeuAc synthetase | - |
Acetivibrio thermocellus |
2.7.7.43 | CSS | - |
Acetivibrio thermocellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 50 | - |
- |
Acetivibrio thermocellus |
2.7.7.43 | 50 | - |
maximum activity | Acetivibrio thermocellus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 37 | 60 | enzyme is active between 37°C - 60°C | Acetivibrio thermocellus |
2.7.7.43 | 37 | 60 | in the presence of 0.2 mM dithiothreitol | Acetivibrio thermocellus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 37 | 70 | complete loss of activity above 70°C, no loss of activity after 24 h incubation at 37°C and 50°C | Acetivibrio thermocellus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 1.66 | - |
NeuGc | - |
Acetivibrio thermocellus | |
2.7.7.43 | 1.66 | - |
N-glycolylneuraminic acid | at 50°C, 10 mM MnCl2, 50 mM Tris buffer (pH 9.5) | Acetivibrio thermocellus | |
2.7.7.43 | 2.25 | - |
NeuAc | - |
Acetivibrio thermocellus | |
2.7.7.43 | 2.25 | - |
N-acylneuraminate | at 50°C, 10 mM MnCl2, 50 mM Tris buffer (pH 9.5) | Acetivibrio thermocellus | |
2.7.7.43 | 3.33 | - |
CTP | - |
Acetivibrio thermocellus | |
2.7.7.43 | 3.33 | - |
CTP | at 50°C, 10 mM MnCl2, 50 mM Tris buffer (pH 9.5) | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 9.5 | - |
- |
Acetivibrio thermocellus |
2.7.7.43 | 9.5 | - |
maximum activity | Acetivibrio thermocellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 8 | 13 | enzyme is active between pH 8-13 | Acetivibrio thermocellus |
2.7.7.43 | 8 | 13 | in Tris-HCl buffer | Acetivibrio thermocellus |