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Literature summary extracted from

  • Fiki, A.E.; Metabteb, G.E.; Bellebna, C.; Wartmann, T.; Bode, R.; Gellissen, G.; Kunze, G.
    The Arxula adeninivorans ATAL gene encoding transaldolase-gene characterization and biotechnological exploitation (2007), Appl. Microbiol. Biotechnol., 74, 1292-1299.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.2.1.2 NaCl increased expression in media containing 5% NaCl Blastobotrys adeninivorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.2
-
 Blastobotrys adeninivorans
2.2.1.2 expressed in Escherichia coli Blastobotrys adeninivorans
2.2.1.2 gene ATAL, DNA and amino acid sequence determination and analysis, including promoter and terminator sequences, single copy gene, genetic organization, expression in Escherichia coli Blastobotrys adeninivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.2 0.013
-
 D-erythrose-4-phosphate
-
 Blastobotrys adeninivorans
2.2.1.2 0.013
-
 D-erythrose 4-phosphate pH 6.0, 20°C Blastobotrys adeninivorans
2.2.1.2 0.056
-
 D-fructose 6-phosphate
-
 Blastobotrys adeninivorans
2.2.1.2 0.056
-
 D-fructose 6-phosphate pH 6.0, 20°C Blastobotrys adeninivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.2 35000
-
 denatured enzyme, SDS-PAGE Blastobotrys adeninivorans
2.2.1.2 35000
-
 4 * 35000, SDS-PAGE Blastobotrys adeninivorans
2.2.1.2 35095
-
 4 * 35000, SDS-PAGE, 4 * 35095, calculated Blastobotrys adeninivorans
2.2.1.2 140000
-
 gel filtration Blastobotrys adeninivorans
2.2.1.2 140000
-
 native enzyme (tetramer), gel filtration Blastobotrys adeninivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate Blastobotrys adeninivorans
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate Blastobotrys adeninivorans LS3
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Blastobotrys adeninivorans
-
-
-
2.2.1.2 Blastobotrys adeninivorans
-
 increased expression in media containing 5% NaCl
-
2.2.1.2 Blastobotrys adeninivorans Q0E4W0 gene ATAL
-
2.2.1.2 Blastobotrys adeninivorans LS3 Q0E4W0 gene ATAL
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.2 additional information
-
-
 Blastobotrys adeninivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 Blastobotrys adeninivorans sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate the preferred substrates for the enzyme include D-erythrose-4-phosphate and D-fructose-6-phosphate, whereas D-glyceraldehyde is not converted Blastobotrys adeninivorans sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 Blastobotrys adeninivorans LS3 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate the preferred substrates for the enzyme include D-erythrose-4-phosphate and D-fructose-6-phosphate, whereas D-glyceraldehyde is not converted Blastobotrys adeninivorans LS3 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.2 D-fructose 6-phosphate + D-erythrose 4-phosphate preferred substrates Blastobotrys adeninivorans sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.2 additional information no substrate: D-glyceraldehyde Blastobotrys adeninivorans ?
-
 ?
2.2.1.2 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 Blastobotrys adeninivorans D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.2.1.2 tetramer
-
 Blastobotrys adeninivorans
2.2.1.2 tetramer 4 * 35000, SDS-PAGE Blastobotrys adeninivorans
2.2.1.2 tetramer 4 * 35000, SDS-PAGE, 4 * 35095, calculated Blastobotrys adeninivorans

Synonyms

EC Number Synonyms Comment Organism
2.2.1.2 ATAL
-
 Blastobotrys adeninivorans
2.2.1.2 Atalp
-
 Blastobotrys adeninivorans
2.2.1.2 transaldolase
-
 Blastobotrys adeninivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.2 20
-
-
 Blastobotrys adeninivorans
2.2.1.2 20
-
 native and recombinant enzyme Blastobotrys adeninivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.2 18 25
-
 Blastobotrys adeninivorans
2.2.1.2 18 25 native and recombinant enzyme, 80% of maximal activity within this range Blastobotrys adeninivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.2 additional information
-
 D-erythrose 4-phosphate pH 6.0, 20°C Blastobotrys adeninivorans
2.2.1.2 4.9
-
 D-fructose 6-phosphate
-
 Blastobotrys adeninivorans
2.2.1.2 4.9
-
 D-fructose 6-phosphate pH 6.0, 20°C Blastobotrys adeninivorans
2.2.1.2 6.44
-
 D-erythrose-4-phosphate
-
 Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.2 5
-
 native enzyme Blastobotrys adeninivorans
2.2.1.2 5.5
-
-
 Blastobotrys adeninivorans
2.2.1.2 5.5
-
 native enzyme Blastobotrys adeninivorans
2.2.1.2 6
-
 recombinant enzyme Blastobotrys adeninivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.2 5 6
-
 Blastobotrys adeninivorans
2.2.1.2 5 6 native enzyme, 80% of maximal activity within this range Blastobotrys adeninivorans
2.2.1.2 5 6 native enzyme Blastobotrys adeninivorans
2.2.1.2 5.4 6.3 recombinant enzyme Blastobotrys adeninivorans
2.2.1.2 5.4 6.3 recombinant enzyme, 80% of maximal activity within this range Blastobotrys adeninivorans