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Literature summary extracted from

  • Biedendieck, R.; Beine, R.; Gamer, M.; Jordan, E.; Buchholz, K.; Seibel, J.; Dijkhuizen, L.; Malten, M.; Jahn, D.
    Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium (2007), Appl. Microbiol. Biotechnol., 74, 1062-1073.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium Limosilactobacillus reuteri

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Limosilactobacillus reuteri
-
-
-
2.4.1.10 Limosilactobacillus reuteri 21
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 purification of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. The gentle one-step purification method for the recombinant protein directly from the cell-free growth medium provides an alternative to Escherichia coli production and purification systems, where cell disruption, several centrifugation steps, and often more than one purification step are necessary. The one-step purification yields comparable amounts of pure and active enzyme Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru Limosilactobacillus reuteri ?
-
 ?
2.4.1.10 alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru Limosilactobacillus reuteri 21 ?
-
 ?
2.4.1.10 alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside only one mannosyloligofructoside is formed Limosilactobacillus reuteri ?
-
 ?
2.4.1.10 alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside only one mannosyloligofructoside is formed Limosilactobacillus reuteri 21 ?
-
 ?
2.4.1.10 alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru Limosilactobacillus reuteri ?
-
 ?
2.4.1.10 alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru Limosilactobacillus reuteri 21 ?
-
 ?