Literature summary extracted from

Miyahisa, I.; Funa, N.; Ohnishi, Y.; Martens, S.; Moriguchi, T.; Horinouchi, S.
Combinatorial biosynthesis of flavones and flavonols in Escherichia coli (2006), Appl. Microbiol. Biotechnol., 71, 53-58.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.11.9	expressed in Escherichia coli strain BLR (DE3)	Citrus sinensis
1.14.20.5	expressed in Escherichia coli BLR (DE3) cells	Petroselinum crispum
2.3.1.74	recombinant Escherichia coli cells containing four genes for a phenylalanine ammonia-lyase, cinnamate/coumarate:CoA ligase, chalcone synthase, and chalcone isomerase, in addition to the acetyl-CoA carboxylase, have been established for efficient production of (2S)-naringenin from tyrosine and (2S)-pinocembrin from phenylalanine. Further introduction of the flavone synthase I gene from Petroselinum crispum under the control of the T7 promoter and the synthetic ribosome-binding sequence in pACYCDuet-1 causes the Escherichia coli cells to produce flavones: apigenin (13 mg/l) from tyrosine and chrysin (9.4 mg/l) from phenylalanine	Glycyrrhiza glabra

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.11.9	Citrus sinensis	Q9ZWR0	-	-
1.14.20.5	Petroselinum crispum	Q7XZQ8	-	-
2.3.1.74	Glycyrrhiza glabra	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.11.9	naringenin + 2-oxoglutarate + O2	-	Citrus sinensis	(2R,3R)-dihydrokaempferol + succinate + CO2	-	?
1.14.20.5	(2S)-naringenin + 2-oxoglutarate + O2	-	Petroselinum crispum	apigenin + succinate + CO2 + H2O	-	?
1.14.20.5	(2S)-pinocembrin + 2-oxoglutarate + O2	-	Petroselinum crispum	chrysin + succinate + CO2 + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.11.9	F3H	-	Citrus sinensis
1.14.11.9	flavanone 3beta-hydroxylase	-	Citrus sinensis
1.14.20.5	flavone synthase I	-	Petroselinum crispum
1.14.20.5	FNS I	-	Petroselinum crispum

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Release 2023.1 (January 2023)