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Literature summary extracted from

  • Suenaga, H.; Goto, M.; Furukawa, K.
    Active-site engineering of biphenyl dioxygenase: effect of substituted amino acids on substrate specificity and regiospecificity (2006), Appl. Microbiol. Biotechnol., 71, 168-176.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 expressed in Escherichia coli Pseudomonas oleovorans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.18 I335F/T376N does not show any significant difference in the oxidation of biphenyl compared with wild type Bph Dox, and exhibits 2,3-dioxygenase activity for 2,2'-dichlorobiphenyl and 3,4-dioxygenase activity for 2,5,4'-trichlorobiphenyl Pseudomonas oleovorans
1.14.12.18 T376D decreased degradation activity Pseudomonas oleovorans
1.14.12.18 T376F shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans
1.14.12.18 T376G decreased degradation activity Pseudomonas oleovorans
1.14.12.18 T376K shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans
1.14.12.18 T376N shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans
1.14.12.18 T376S decreased degradation activity Pseudomonas oleovorans
1.14.12.18 T376V shows novel degradation activity for dibenzofuran, which is a poor substrate for the wild type enzyme Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Pseudomonas oleovorans
-
-
-
1.14.12.18 Pseudomonas oleovorans KF707
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+
-
 ?
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans KF707 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+
-
 ?
1.14.12.18 2,5,4'-trichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.12.18 2,5,4'-trichlorobiphenyl + NADH + O2
-
 Pseudomonas oleovorans KF707 ?
-
 ?
1.14.12.18 4,4'-dichlorobiphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans 2,3-dihydroxy-4,4'-dichlorobiphenyl + NAD(P)+
-
 ?
1.14.12.18 4,4'-dichlorobiphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans KF707 2,3-dihydroxy-4,4'-dichlorobiphenyl + NAD(P)+
-
 ?
1.14.12.18 4-methylbiphenyl + NADH + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxo-6-[4-methylphenyl]-hexa-2,4-dienoic acid + NAD+
-
 ?
1.14.12.18 4-methylbiphenyl + NADH + O2
-
 Pseudomonas oleovorans KF707 2-hydroxy-6-oxo-6-[4-methylphenyl]-hexa-2,4-dienoic acid + NAD+
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Pseudomonas oleovorans KF707 cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 dibenzofuran + NADH + O2 dibenzofuran is a poor substrate Pseudomonas oleovorans ?
-
 ?
1.14.12.18 diphenylmethane + NADH + O2
-
 Pseudomonas oleovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 biphenyl dioxygenase
-
 Pseudomonas oleovorans
1.14.12.18 BPH dox
-
 Pseudomonas oleovorans
1.14.12.18 BphABC
-
 Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 NADH
-
 Pseudomonas oleovorans
1.14.12.18 NADPH
-
 Pseudomonas oleovorans