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Literature summary extracted from

  • Dib, I.; Stanzer, D.; Nidetzky, B.
    Trigonopsis variabilis D-amino aacid oxidase: control of protein quality and opportunities for biocatalysis through production in Escherichia coli (2007), Appl. Environ. Microbiol., 73, 331-333.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.3 additional information exogenous FAD (0.01 to 1 mM) added to assay or protein storage buffers does not enhance the catalytic activity of native and recombinant DAO Trigonopsis variabilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli BL21(DE3) cells Trigonopsis variabilis
1.4.3.3 expression in Escherichia coli. TvDAO can be produced in Escherichia coli as a fully functional recombinant protein. They also show that microheterogeneity due to modification of Cys108 is avoided completely during production and purification Trigonopsis variabilis

General Stability

EC Number General Stability Organism
1.4.3.3 oriented immobilization of a chimeric oxidase maintains 80% of the original activity in microparticle-bound enzymes. Mildly permeabilized cells in which N-terminally tagged TvDAO in which a 12-amino-acid peptide is fused in frame to the N-terminus (TvDAOstrepN) is physically entrapped are employed for the batchwise conversion of D-Ala in an aerated enzyme reactor. The cells could be reused for at least three rounds of reaction with only small losses of activity. The free enzyme, however, is rapidly inactivated under the same reaction conditions (half-life: 1.2 h) Trigonopsis variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 additional information resistant to the oxidants O2 and H2O2 Trigonopsis variabilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Trigonopsis variabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Trigonopsis variabilis
1.4.3.3 MonoQ column chromatography Trigonopsis variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Trigonopsis variabilis 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Trigonopsis variabilis pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Trigonopsis variabilis 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Trigonopsis variabilis
1.4.3.3 DAO
-
 Trigonopsis variabilis
1.4.3.3 TvDAO
-
 Trigonopsis variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 30
-
 increasing the temperature to 30°C does not affect the specific activity but enhances the volumetric enzyme activity by 22% due to its effect on cell growth Trigonopsis variabilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Trigonopsis variabilis