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Literature summary extracted from
- Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion (2005), Appl. Environ. Microbiol., 71, 8873-8880.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.26 | expressed in Escherichia coli Tuner(DE3) and BL21(DE3) cells | Streptomyces clavuligerus |
| 1.14.20.1 | expressed in Escherichia coli | Streptomyces clavuligerus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.11.26 | A106T | 80% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | C155Y | 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | C155Y/Y184H/V275I/C281Y | 580% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | C281Y | 200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | G300V | 410% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | G79E | 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | H244Q | 140% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | I305L | 230% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | I305M | 380% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | L277Q | 270% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | M188I | 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | M188V | 150% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | M73T | 180% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | N304K | 220% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | T91A | 110% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | V275I | 270% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | V275I/I305M | 500% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.11.26 | Y184H | 200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.20.1 | A106T | 80% relative activity with 1 mM substrate and 170% relative activity with 10 mM penicillin G compared to wild-type activity detected with 1 mM penicillin G, 1.5fold reduction in Km, penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.20.1 | A106T/C155Y | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | A106T/M188V | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | A11V/T91A/C281Y/I305L | 220% relative activity with 1 mM and 200% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | C155Y | 90% relative activity with 1 mM and 180% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, 1.5fold reduction in Km, penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.20.1 | C155Y/Y184H/V275I/C281Y | 580% relative activity with 1 mM and 670% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | C281R | activity similar to wild-type enzyme | Streptomyces clavuligerus |
| 1.14.20.1 | C281Y | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | D107G | activity similar to wild-type enzyme | Streptomyces clavuligerus |
| 1.14.20.1 | D107G/L277Q | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | D53H/C281Y | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | E144K/M188I/A198T/V275I/C281Y | 160% relative activity with 1 mM and 200% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | G300V | 410% relative activity with 1 mM and 370% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | G300V | substrate inhibition, epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | H244Q | 140% relative activity with 1 mM and 270% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, 1.9fold reduction in Km, penicillin G as substrate, epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | I305L | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | L277Q | 270% relative activity with 1 mM and 410% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, 2.5fold reduction in Km, penicillin G as substrate | Streptomyces clavuligerus |
| 1.14.20.1 | L277Q/I305M | 610% relative activity with 1 mM and 520% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | M188I | 90% relative activity with 1 mM and 190% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | M188V | 150% relative activity with 1 mM and 190% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | M188V/V275I/G300V | 440% relative activity with 1 mM and 310% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | M73T | 180% relative activity with 1 mM and 250% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, 3.5fold reduction in Km for penicillin N and 2.3fold reduction in Km for penicillin G, epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | M73T/C281Y | 610% relative activity with 1 mM and 530% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | M73T/G300V/I305L | 640% relative activity with 1 mM and 380% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | M73T/I118V/A140V/H244Q/C281Y | 450% relative activity with 1 mM and 350% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | M73T/I277Q | 350% relative activity with 1 mM and 350% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | M73T/P145L | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | R135Q/C155Y/R179Q/M188V/I305M | 360% relative activity with 1 mM and 290% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | T91A | 110% relative activity with 1 mM and 200% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, 1.9fold reduction in Km, penicillin G as substrate, epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | V275I/C281Y | 260% relative activity with 1 mM and 310% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | V275I/C281Y/G300V | 620% relative activity with 1 mM and 380% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | V275I/I305L | 310% relative activity with 1 mM and 330% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | Y184H | 200% relative activity with 1 mM and 330% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | Y184H/C281R | epPCR random mutagenesis | Streptomyces clavuligerus |
| 1.14.20.1 | Y184H/H244Q/T259I/L277Q | 320% relative activity with 1 mM and 310% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | Y184H/M188I/C281Y/I305L | 610% relative activity with 1 mM and 460% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.26 | 0.004 | - |
Penicillin N | mutant enzyme N304K | Streptomyces clavuligerus |  |
| 1.14.11.26 | 0.006 | - |
Penicillin N | mutant enzyme C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.006 | - |
Penicillin N | mutant enzyme I305L | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.006 | - |
Penicillin N | mutant enzyme M73T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.009 | - |
Penicillin N | mutant enzyme G79E | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.009 | - |
Penicillin N | mutant enzyme H244Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.009 | - |
Penicillin N | mutant enzyme T91A | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.011 | - |
Penicillin N | mutant enzyme L277Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.011 | - |
Penicillin N | mutant enzyme M188I | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.012 | - |
Penicillin N | mutant enzyme I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.012 | - |
Penicillin N | mutant enzyme V275I | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.013 | - |
Penicillin N | mutant enzyme A106T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.013 | - |
Penicillin N | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.014 | - |
Penicillin N | wild type enzyme | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.016 | - |
Penicillin N | mutant enzyme C155Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.047 | - |
Penicillin N | mutant enzyme M188V | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.092 | - |
Penicillin N | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.19 | - |
penicillin G | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.22 | - |
penicillin G | mutant enzyme N304K | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.25 | - |
penicillin G | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.295 | - |
Penicillin N | mutant enzyme Y184H | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.66 | - |
penicillin G | mutant enzyme I305L | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.68 | - |
penicillin G | mutant enzyme C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.74 | - |
penicillin G | mutant enzyme M73T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.75 | - |
penicillin G | mutant enzyme G79E | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.75 | - |
penicillin G | mutant enzyme I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.76 | - |
penicillin G | mutant enzyme M188V | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.95 | - |
penicillin G | mutant enzyme Y184H | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.02 | - |
penicillin G | mutant enzyme L277Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.36 | - |
penicillin G | mutant enzyme T91A | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.39 | - |
penicillin G | mutant enzyme H244Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.68 | - |
penicillin G | mutant enzyme V275I | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.76 | - |
penicillin G | mutant enzyme C155Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.77 | - |
penicillin G | mutant enzyme A106T | Streptomyces clavuligerus | |
| 1.14.11.26 | 1.96 | - |
penicillin G | mutant enzyme M188I | Streptomyces clavuligerus | |
| 1.14.11.26 | 2.58 | - |
penicillin G | wild type enzyme | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.006 | - |
Penicillin N | mutant M73T | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.009 | - |
Penicillin N | mutant H244Q | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.009 | - |
Penicillin N | mutant T91A | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.011 | - |
Penicillin N | mutant L277Q | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.011 | - |
Penicillin N | mutant M188I | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.013 | - |
Penicillin N | mutant A106T | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.014 | - |
Penicillin N | wild-type | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.016 | - |
Penicillin N | mutant C155Y | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.047 | - |
Penicillin N | mutant M188V | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.092 | - |
Penicillin N | mutant C155Y/Y184H/V275I/C281Y | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.19 | - |
penicillin G | mutant C155Y/Y184H/V275I/C281Y | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.295 | - |
Penicillin N | mutant Y184H | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.74 | - |
penicillin G | mutant M73T | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.76 | - |
penicillin G | mutant M188V | Streptomyces clavuligerus | |
| 1.14.20.1 | 0.95 | - |
penicillin G | mutant Y184H | Streptomyces clavuligerus | |
| 1.14.20.1 | 1.02 | - |
penicillin G | mutant L277Q | Streptomyces clavuligerus | |
| 1.14.20.1 | 1.36 | - |
penicillin G | mutant T91A | Streptomyces clavuligerus | |
| 1.14.20.1 | 1.39 | - |
penicillin G | mutant H244Q | Streptomyces clavuligerus | |
| 1.14.20.1 | 1.76 | - |
penicillin G | mutant C155Y | Streptomyces clavuligerus | |
| 1.14.20.1 | 1.77 | - |
penicillin G | mutant A106T | Streptomyces clavuligerus | |
| 1.14.20.1 | 1.96 | - |
penicillin G | mutant M188I | Streptomyces clavuligerus | |
| 1.14.20.1 | 2.58 | - |
penicillin G | wild-type | Streptomyces clavuligerus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.26 | Fe2+ | Fe2+-dependent | Streptomyces clavuligerus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.26 | penicillin N + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
? + succinate + CO2 | - |
? | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.26 | Streptomyces clavuligerus | - |
- |
- |
| 1.14.20.1 | Streptomyces clavuligerus | P18548 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.26 | anion-exchange chromatography and gel filtration | Streptomyces clavuligerus |
| 1.14.20.1 | anion-exchange and gel filtration | Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | additional information | - |
160% relative activity with 10 mM substrate penicillin G compared to the activity detected with 1 mM penicillin G | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.26 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetylcephalosporin C + succinate + CO2 | - |
? | |
| 1.14.11.26 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
| 1.14.11.26 | penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
| 1.14.11.26 | phenylacetyl-7-aminodeacetoxycephalosporanic acid + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetylcephalosporin + succinate + CO2 | - |
? | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.26 | DAOCS | - |
Streptomyces clavuligerus |
| 1.14.11.26 | deacetoxycephalosporin C synthase | - |
Streptomyces clavuligerus |
| 1.14.20.1 | DAOCS | - |
Streptomyces clavuligerus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.26 | 0.0315 | - |
penicillin G | mutant enzyme G79E | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0453 | - |
penicillin G | wild type enzyme | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0453 | - |
penicillin G | mutant enzyme A106T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0456 | - |
penicillin G | mutant enzyme M188V | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0476 | - |
penicillin G | mutant enzyme C155Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.048 | - |
Penicillin N | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.048 | - |
Penicillin N | mutant enzyme Y184H | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0502 | - |
penicillin G | mutant enzyme V275I | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0507 | - |
penicillin G | mutant enzyme M188I | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0522 | - |
penicillin G | mutant enzyme T91A | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0564 | - |
penicillin G | mutant enzyme N304K | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0627 | - |
penicillin G | mutant enzyme M73T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0698 | - |
penicillin G | mutant enzyme H244Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0744 | - |
penicillin G | mutant enzyme C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0759 | - |
penicillin G | mutant enzyme I305L | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.0798 | - |
penicillin G | mutant enzyme Y184H | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.084 | - |
Penicillin N | mutant enzyme M188V | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.1036 | - |
penicillin G | mutant enzyme L277Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.1398 | - |
penicillin G | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.1452 | - |
penicillin G | mutant enzyme I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.1458 | - |
penicillin G | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.178 | - |
Penicillin N | mutant enzyme G79E | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.235 | - |
Penicillin N | mutant enzyme C155Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.239 | - |
Penicillin N | mutant enzyme M73T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.247 | - |
Penicillin N | mutant enzyme T91A | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.252 | - |
Penicillin N | mutant enzyme V275I | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.26 | - |
Penicillin N | mutant enzyme H244Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.273 | - |
Penicillin N | mutant enzyme C281Y | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.277 | - |
Penicillin N | mutant enzyme A106T | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.277 | - |
Penicillin N | mutant enzyme M188I | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.284 | - |
Penicillin N | mutant enzyme I305L | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.297 | - |
Penicillin N | mutant enzyme L277Q | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.307 | - |
Penicillin N | wild type enzyme | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.31 | - |
Penicillin N | mutant enzyme I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.316 | - |
Penicillin N | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
| 1.14.11.26 | 0.366 | - |
Penicillin N | mutant enzyme N304K | Streptomyces clavuligerus | |
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