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Literature summary extracted from

  • Al-Saeedi, F.; Smith, T.; Welch, A.
    [Methyl-3H]-choline incorporation into MCF-7 cells: correlation with proliferation, choline kinase and phospholipase D assay (2007), Anticancer Res., 27, 901-906.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.32 additional information activation of choline kinase is essential for building membranes, cell growth, cell proliferationand for rebuilding phospholipids that are degraded in the process of signal transductiony Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.32 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.32 choline + ATP Homo sapiens
-
 phosphocholine + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 breast cancer cell
-
 Homo sapiens
-
2.7.1.32 MCF-7 cell
-
 Homo sapiens
-
2.7.1.32 MCF-7 cell human breast cancer cell line Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.32 additional information
-
 exponentially growing cells: 484.04 pmol/mg/min (activity is determined after homogenizing a cell pellet in Tris/HCl buffer. Homogenisate of exponentially growing and confluent cells is centrifugated. Assay mixture containing 40 mM Tris/HCl with unlabelled carrier of 0.25 mM choline, (methyl-3H)choline (37 kBq/sample), 2 mM ATP and 10 mM MgCl2 were added to homogenate protein. After incubation, reaction is stopped by addition of ice-cold mixture of methanol and chloroform and lipid-metabolite extraction is then carried out. Protein content is set for each sample and total radioactivity is determined. Separation of (methyl-3H)-phosphocholine and (methyl-3H)-choline is carried out using ion exchange chromatography) Homo sapiens
2.7.1.32 0.000484
-
 in 40 mM Tris/HCl with 10 mM MgCl2, 2 mM ATP and 0.25 mM choline, pH 7.4, at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Homo sapiens ADP + phosphocholine
-
 ?
2.7.1.32 choline + ATP
-
 Homo sapiens phosphocholine + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 ChoK
-
 Homo sapiens
2.7.1.32 choline kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.32 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.32 ATP
-
 Homo sapiens