EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.99.12 | drug development | the enzyme is a target in antimicrobial inhibitor development, structure-based inhibitor design | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.12 | subcloning in strain DH5alpha, expression in strain BL21(DE3) | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.12 | Mg2+ | required, cannot be substituted by Ca2+, KM: 0.99 mM | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.99.12 | 23177 | - |
x * 23177, mass spectrometry | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.12 | D-ribulose 5-phosphate | Escherichia coli | step in biosynthesis of vitamin B2, riboflavin | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
4.1.99.12 | D-ribulose 5-phosphate | Escherichia coli W3110 / ATCC 27325 | step in biosynthesis of vitamin B2, riboflavin | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.12 | Escherichia coli | - |
- |
- |
4.1.99.12 | Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.12 | recombinant enzyme from strain Bl21(DE3) by hydrophobic interaction and anion exchange chromatography, and ultrafiltration | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.99.12 | additional information | - |
development of a spectrophotometric/colorimetric assay method, overview | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.12 | D-ribulose 5-phosphate | - |
Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
4.1.99.12 | D-ribulose 5-phosphate | step in biosynthesis of vitamin B2, riboflavin | Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
4.1.99.12 | D-ribulose 5-phosphate | - |
Escherichia coli W3110 / ATCC 27325 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
4.1.99.12 | D-ribulose 5-phosphate | step in biosynthesis of vitamin B2, riboflavin | Escherichia coli W3110 / ATCC 27325 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.99.12 | ? | x * 23177, mass spectrometry | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.12 | 25 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.99.12 | 7.5 | - |
assay at | Escherichia coli |