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Literature summary extracted from

  • Pietila, M.P.; Vohra, P.K.; Sanyal, B.; Wengenack, N.L.; Raghavakaimal, S.; Thomas, C.F.
    Cloning and characterization of CYP51 from Mycobacterium avium (2006), Am. J. Respir. Cell Mol. Biol., 35, 236-242.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.154 medicine the enzyme is a target in treatment of the chronic lung disease caused by Mycobacterium avium Mycobacterium avium subsp. avium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expression in Escherichia coli as GST-tagged enzyme Mycobacterium avium subsp. avium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 econazole
-
 Mycobacterium avium subsp. avium
1.14.14.154 fluconazole
-
 Mycobacterium avium subsp. avium
1.14.14.154 itraconazole
-
 Mycobacterium avium subsp. avium
1.14.14.154 ketoconazole
-
 Mycobacterium avium subsp. avium
1.14.14.154 voriconazole
-
 Mycobacterium avium subsp. avium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.154 Iron
-
 Mycobacterium avium subsp. avium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Mycobacterium avium subsp. avium
-
 ?
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 Mycobacterium avium subsp. avium preferred substrate 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Mycobacterium avium subsp. avium Q3HTM1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.154 recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, cleavage of the tag by thrombin, and thrombin elimination by benzamidine affinity chromatography Mycobacterium avium subsp. avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Mycobacterium avium subsp. avium ?
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 preferred substrate Mycobacterium avium subsp. avium 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
 Mycobacterium avium subsp. avium
1.14.14.154 lanosterol 14alpha-demethylase
-
 Mycobacterium avium subsp. avium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.154 37
-
 assay at Mycobacterium avium subsp. avium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.154 7.4
-
 assay at Mycobacterium avium subsp. avium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.154 Ferredoxin
-
 Mycobacterium avium subsp. avium