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Literature summary extracted from

  • Meier, C.; Carter, L.G.; Winter, G.; Owens, R.J.; Stuart, D.I.; Esnouf, R.M.
    Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis (BA4489) (2007), Acta Crystallogr. Sect. F, F63, 168-172.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.2 expressed in Escherichia coli (strain Rosetta pLysS) Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.3.2 cocrystallization with folinate, ATP and Mg2+, sitting drop vapour diffusion method using 25% polyethylene glycol 3350, 100 mM bis-Tris pH 5.5 Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.3.2 Mg2+ dependent Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Bacillus anthracis Q81LX0
-
-
6.3.3.2 Bacillus anthracis BA4489 Q81LX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2 Ni-NTA affinity chromatography and gel filtration Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Bacillus anthracis ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Bacillus anthracis BA4489 ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.2 dimer x-ray crystallography Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
6.3.3.2 5,10-Methenyltetrahydrofolate synthetase
-
 Bacillus anthracis
6.3.3.2 MTHFS
-
 Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.3.2 ATP
-
 Bacillus anthracis