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Literature summary extracted from

  • Muench, S.P.; Prigge, S.T.; Zhu, L.; Kirisits, M.J.; Roberts, C.W.; Wernimont, S.; McLeod, R.; Rice, D.W.
    Expression, purification and preliminary crystallographic analysis of the Toxoplasma gondii enoyl reductase (2006), Acta Crystallogr. Sect. F, F62, 604-606.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.1.9 medicine antibacterial target Toxoplasma gondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 into the pMALc2x vector without signal peptide coding sequence, a second construct contains in addition the sequence of the TEV protease cleaving site followed by a hexahistidine tag Toxoplasma gondii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.9 crystals of enoyl reductase in complex with NAD+ and triclosan, data are collected to beyond 2.6 A Toxoplasma gondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 triclosan
-
 Toxoplasma gondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.9 acyl-[acyl-carrier protein] + NAD+ Toxoplasma gondii
-
 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 using a HiTrap Chelating HP and an amylose column Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 acyl-[acyl-carrier protein] + NAD+
-
 Toxoplasma gondii trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl reductase
-
 Toxoplasma gondii
1.3.1.9 enoyl-[acyl-carrier-protein] reductase
-
 Toxoplasma gondii
1.3.1.9 ENR
-
 Toxoplasma gondii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Toxoplasma gondii