EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.6 | expressed in Escherichia coli strain BL21 codon plus (DE3)-RIL | Pyrococcus horikoshii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.6 | seeding method in combination with the sitting-drop vapour-diffusion method using 30%(v/v) PEG 400, 1 M D-galactose, 5 mM AMP-PNP, 5% (w/v) PEG 4000, 50 mM MgCl2 and 0.1 M MES pH 6.3. | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.132 | - |
ATP | in 50 mM HEPES-NaOH pH 7.5, at 50°C | Pyrococcus horikoshii | |
2.7.1.6 | 0.403 | - |
D-galactose | in 50 mM HEPES-NaOH pH 7.5, at 50°C | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.6 | Pyrococcus horikoshii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.6 | HiTrap Q column chromatography, HiTrap Phenyl FF column chromatography, and HiLoad 16/60 Superdex 75 gel filtration | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.6 | ATP + D-galactose | - |
Pyrococcus horikoshii | ADP + alpha-D-galactose 1-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.6 | GALK | - |
Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.6 | 37 | - |
very little activity at 37°C | Pyrococcus horikoshii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.91 | - |
ATP | in 50 mM HEPES-NaOH pH 7.5, at 50°C | Pyrococcus horikoshii | |
2.7.1.6 | 0.91 | - |
D-galactose | in 50 mM HEPES-NaOH pH 7.5, at 50°C | Pyrococcus horikoshii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.6 | ATP | - |
Pyrococcus horikoshii |