EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.267 | drug development | the enzyme is an excellent drug target in a number of pathogenic organisms | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.267 | gene Rv2870c, expression of His-tagged enzyme in Escherichia coli strain BL21 | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.267 | purified recombinant His-tagged enzyme with inhibitor fosmidomycin, microbatch method, sitting drops mixed from 0.001 ml of protein solution, containing 5 mg/ml protein in 150 mM NaCl and 20 mM TrisHCl pH 7.5, and 0.001 ml of crystallization buffer, containing 40% ethylene glycol, 2 mM MgSO4, 20 mM DTT, 0.2 mM EDTA and 100 mM acetate pH 5.0, streak-seeded immediately with a horse hair, 4 weeks, stabilization prior to flash-cooling in reservoir solution complemented with additional 10% ethylene glycol and 1 mM fosmidomycin, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | fosmidomycin | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.267 | 0.0072 | - |
NADPH | - |
Mycobacterium tuberculosis | |
1.1.1.267 | 0.34 | - |
1-deoxy-D-xylulose 5-phosphate | - |
Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | Mn2+ | required | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis H37Rv | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.267 | Mycobacterium tuberculosis | - |
gene Rv2870c | - |
1.1.1.267 | Mycobacterium tuberculosis H37Rv | - |
gene Rv2870c | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.267 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal ion affinity chromatography, and gel filtration, to 98% purity | Mycobacterium tuberculosis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.267 | 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | active site structure | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.267 | dimer | enzyme crystal structure, the dimer interface is created by interactions between the catalytic and connecting regions of each subunit, a sulfate is bound at the expected site of the phosphate moiety of the sugar substrate, molecular replacement structure analysis and comparison, overview | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.267 | DXR | - |
Mycobacterium tuberculosis |
1.1.1.267 | Rv2870c | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.267 | 22 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.267 | 5.3 | - |
NADPH | - |
Mycobacterium tuberculosis | |
1.1.1.267 | 5.3 | - |
1-deoxy-D-xylulose 5-phosphate | - |
Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.267 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | NADPH | - |
Mycobacterium tuberculosis |