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Literature summary extracted from

  • Do, Y.H.; Gifford-Moore, D.S.; Beight, D.W.; Rathnachalam, R.; Klimkowski, V.J.; Warshawsky, A.M.; Lu, D.
    Inhibition of thrombin activatable fibrinolysis inhibitor by cysteine derivatives (2005), Thromb. Res., 116, 265-271.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.20 DL-mercaptomethyl-3-guanidinoethylthiopropanoic acid i.e. Plummer's inhibitor, IC50: 0.029 mM Homo sapiens
3.4.17.20 glycyl-glycyl-L-cysteine
-
 Homo sapiens
3.4.17.20 glycyl-L-cysteine
-
 Homo sapiens
3.4.17.20 guanidinyl-L-cysteine IC50: 0.0094 mM in inhibition of plasma fibrin clot lysis Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.20 Zn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.20 fibrin + H2O Homo sapiens partially degraded fibrin in fibrin clots, cleavage of C-terminal Lys fibrin + Lys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.20 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.20 proteolytic modification Ca2+-dependent activation by thrombin at room temperature Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.17.20 release of C-terminal Arg and Lys from a polypeptide catalytically important residues are G207, I243, I247, S254, and I255, catalytic mechanism and substrate binding Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.20 fibrin + H2O partially degraded fibrin in fibrin clots, cleavage of C-terminal Lys Homo sapiens fibrin + Lys
-
 ?
3.4.17.20 [3-(2-furylacryloyl)]-L-alanyl-L-arginine + H2O
-
 Homo sapiens [3-(2-furylacryloyl)]-L-alanine + L-arginine
-
 ?
3.4.17.20 [3-(2-furylacryloyl)]-L-phenylalanyl-L-phenylalanine + H2O
-
 Homo sapiens [3-(2-furylacryloyl)]-L-phenylalanine + L-phenylalanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.20 More active site ligand complex model building using D-Cys, guanidinyl-L-cysteine, glycyl-glycyl-L-cysteine, and glycyl-L-cysteine Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.17.20 TAFI
-
 Homo sapiens
3.4.17.20 thrombin activatable fibrinolysis inhibitor
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.20 22
-
 assay at room temperatur Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.17.20 additional information
-
 additional information inhibition kinetics Homo sapiens
3.4.17.20 0.00008
-
 guanidinyl-L-cysteine 22°C Homo sapiens
3.4.17.20 0.0001
-
 DL-mercaptomethyl-3-guanidinoethylthiopropanoic acid 22°C Homo sapiens
3.4.17.20 0.00014
-
 glycyl-L-cysteine 22°C Homo sapiens
3.4.17.20 0.00099
-
 glycyl-glycyl-L-cysteine 22°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.17.20 0.0094
-
 IC50: 0.0094 mM in inhibition of plasma fibrin clot lysis Homo sapiens guanidinyl-L-cysteine
3.4.17.20 0.029
-
 i.e. Plummer's inhibitor, IC50: 0.029 mM Homo sapiens DL-mercaptomethyl-3-guanidinoethylthiopropanoic acid