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Literature summary extracted from

  • Zhu, D.; Stearns, J.E.; Ramirez, M.; Hua, L.
    Enzymatic enantioselective reduction of alpha-ketoesters by a thermostable 7alpha-hydroxysteroid dehydrogenase from Bacteroides fragilis (2006), Tetrahedron, 62, 4535-4539.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.159 overexpression in Escherichia coli Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.159 7-keto-lithocholic acid + NADH Bacteroides fragilis in the gastrointestinal tract of host organisms cheno-deoxycholic acid + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Bacteroides fragilis
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 strain ATCC 25285
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159 recombinant enzyme from Escherichia coli by heat treatment at 55-60°C, polyethyleneimine treatment, ammonium sulfate fractionation, and gel filtration, followed by lyophilization Bacteroides fragilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 0.32
-
 purified recombinant enzyme, substrate methyl benzoylformate Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 7-keto-lithocholic acid + NADH
-
 Bacteroides fragilis cheno-deoxycholic acid + NAD+
-
 ?
1.1.1.159 7-keto-lithocholic acid + NADH in the gastrointestinal tract of host organisms Bacteroides fragilis cheno-deoxycholic acid + NAD+
-
 ?
1.1.1.159 ethyl (R)-2-hydroxy-2-(3,5-difluorophenyl)acetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-(3,5-difluorophenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl (R)-2-hydroxy-3,3-dimethylbutyrate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-3,3-dimethylbutyrate + NADH
-
 ?
1.1.1.159 ethyl (R)-2-hydroxy-3-methylbutyrate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-3-methylbutyrate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-(3,4-dichlorophenyl)acetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-(3,4-dichlorophenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-(4-bromophenyl)acetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-(4-bromophenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-(4-chlorophenyl)acetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-(4-chlorophenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-(4-cyanophenyl)acetate + NAD+ high activity Bacteroides fragilis ethyl 2-oxo-2-(4-cyanophenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-(4-fluorophenyl)acetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-(4-fluorophenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-(4-methylphenyl)acetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-(4-methylphenyl)acetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-cyclohexylacetate + NAD+ best substrate Bacteroides fragilis ethyl 2-oxo-2-cyclohexylacetate + NADH
-
 ?
1.1.1.159 ethyl 2-hydroxy-2-phenylacetate + NAD+
-
 Bacteroides fragilis ethyl 2-oxo-2-phenylacetate + NADH
-
 ?
1.1.1.159 methyl 2-hydroxy-2-phenylacetate + NAD+
-
 Bacteroides fragilis methyl 2-oxo-2-phenylacetate + NADH
-
 ?
1.1.1.159 methyl benzoylformate + NADH
-
 Bacteroides fragilis ?
-
 ?
1.1.1.159 additional information substrate specificity and stereoselectivity towards various ketones, product determination, overview Bacteroides fragilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.159 7-HSDH
-
 Bacteroides fragilis
1.1.1.159 7alpha-hydroxysteroid dehydrogenase
-
 Bacteroides fragilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.159 22
-
 assay at room temperature Bacteroides fragilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.159 additional information
-
 the enzyme is thermostable Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 7
-
 assay at Bacteroides fragilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NADH
-
 Bacteroides fragilis