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Literature summary extracted from

  • Frey, M.; Chomet, P.; Glawischnig, E.; Stettner, C.; Grun, S.; Winklmair, A.; Eisenreich, W.; Bacher, A.; Meeley, R.B.; Briggs, S.P.; Simcox, K.; Gierl, A.
    Analysis of a chemical plant defense mechanism in grasses (1997), Science, 277, 696-699.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.109 expressed in Saccharomyces cerevisiae WAT11 cells Zea mays
1.14.14.153 expressed in Saccharomyces cerevisiae WAT11 cells Zea mays
1.14.14.157 expressed in Saccharomyces cerevisiae WAT11 cells Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.109 microsome
-
 Zea mays
-
-
1.14.14.153 microsome
-
 Zea mays
-
-
1.14.14.157 microsome
-
 Zea mays
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.8 indole-3-glycerol phosphate Zea mays branch point from tryptophan biosynthesis, the enzyme is required for biosynthesis of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one indole + glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.109 Zea mays Q43250
-
-
1.14.14.153 Zea mays Q43257 hybrid Blizzard
-
1.14.14.157 Zea mays Q43255
-
-
4.1.2.8 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.8 seed
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.109 3-hydroxyindolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.153 indole + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays indolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.157 indolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays 3-hydroxyindolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
4.1.2.8 indole-3-glycerol phosphate
-
 Zea mays indole + glyceraldehyde 3-phosphate
-
 ?
4.1.2.8 indole-3-glycerol phosphate branch point from tryptophan biosynthesis, the enzyme is required for biosynthesis of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one Zea mays indole + glyceraldehyde 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.109 BX4
-
 Zea mays
1.14.14.153 BX2
-
 Zea mays
1.14.14.157 BX3
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.109 cytochrome P450
-
 Zea mays
1.14.14.153 cytochrome P450 dependent on Zea mays
1.14.14.157 cytochrome P450 dependent on Zea mays
1.14.14.157 NADPH
-
 Zea mays