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Literature summary extracted from

  • Spinelli, S.L.; Consaul, S.A.; Phizicky, E.M.
    A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction (1997), RNA, 3, 1388-1400.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.160 Escherichia coli
-
-
-
2.7.1.160 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.160 partially purified by Blue sepharose column chromatography Escherichia coli
2.7.1.160 partially purified, GST-Tpt1 fusion protein purified by glutahthione agarose chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+
-
 Escherichia coli mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+ tRNA_Leu_UAG, tRNA_SER, tRNA_LEU_CAA, tRNA_TYR, tRNA_LYS, tRNA_PRO1, tRNA_PRO2, tRNA_PHE, unidentified tRNA Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.160 Tpt1
-
 Escherichia coli
2.7.1.160 Tpt1
-
 Saccharomyces cerevisiae