EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.160 | expression from Saccharomyces cerevisiae vectors | Saccharomyces cerevisiae |
2.7.1.160 | expression in Saccharomyces cerevisiae | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.160 | F72A | temperature sensitive | Saccharomyces cerevisiae |
2.7.1.160 | H117A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | H117A-T119A | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | H142A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | H22A | lethal in vivo | Escherichia coli |
2.7.1.160 | H24A | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | H24N | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | H24Q | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | H90A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | K16A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | K69A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | R125A | lethal in vivo | Escherichia coli |
2.7.1.160 | R138A | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R138K | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | R138Q | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R158A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | R21A | lethal in vivo | Escherichia coli |
2.7.1.160 | R23A | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R23K | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R23Q | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R69A | lethal in vivo | Escherichia coli |
2.7.1.160 | R71A | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R71K | lethal in vivo | Saccharomyces cerevisiae |
2.7.1.160 | R71Q | grows slower than wild-type, temperature sensitive | Saccharomyces cerevisiae |
2.7.1.160 | S91A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | T119A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
2.7.1.160 | Y38A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.160 | 25000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.160 | Aeropyrum pernix | - |
- |
- |
2.7.1.160 | Archaeoglobus fulgidus | - |
- |
- |
2.7.1.160 | Clostridium perfringens | - |
- |
- |
2.7.1.160 | Drosophila melanogaster | - |
- |
- |
2.7.1.160 | Escherichia coli | - |
- |
- |
2.7.1.160 | Homo sapiens | - |
- |
- |
2.7.1.160 | Leishmania major | - |
- |
- |
2.7.1.160 | Nostoc punctiforme | - |
- |
- |
2.7.1.160 | Pyrococcus horikoshii | - |
- |
- |
2.7.1.160 | Saccharomyces cerevisiae | - |
- |
- |
2.7.1.160 | Schizosaccharomyces pombe | - |
- |
- |
2.7.1.160 | Trypanosoma cruzi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.160 | Ni-agarose chromatography | Escherichia coli |
2.7.1.160 | Ni-agarose chromatography | Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.160 | -80, Tris-HCl buffer, pH 8.0, 10% glycerol | Saccharomyces cerevisiae |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.160 | monomer | SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.160 | KptA | - |
Escherichia coli |
2.7.1.160 | Tpt1 | - |
Saccharomyces cerevisiae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.160 | 18 | 37 | growth of mutants K16A, K69A, H90A, S91A, H117A, T119A, H142A, R158A and R138K on yeast medium/peptone/dextrose agar, colony sizes were similar to that of wild-type TPT1 cells | Saccharomyces cerevisiae |
2.7.1.160 | 18 | 30 | F27A, R71Q, TPT1(1-220) and TPT1(1-210) cells failed to grow at 37°C on yeast medium/peptone/dextrose agar, R71Q and TPT1(1-210) strains grew slower than wild-type TPT1 | Saccharomyces cerevisiae |
2.7.1.160 | 25 | 30 | growth of mutants K16A, Y38A, K69A, F72A, H90A, S91A, H117A, T119A, H142A, R158A, TPT1(1-220) and TPT1(1-210) on 5-fluoroorotic acid agar medium | Saccharomyces cerevisiae |