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Literature summary extracted from

  • Yan, J.B.; Wang, G.Q.; Du, P.; Zhu, D.X.; Wang, M.W.; Jiang, X.Y.
    High-level expression and purification of Escherichia coli oligopeptidase B (2006), Protein Expr. Purif., 47, 645-650.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37°C to 30°C Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.83 recombinant Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 Nalpha-benzoyl-Arg-7-amido-4-methylcoumarin + H2O 68% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide Escherichia coli ?
-
 ?
3.4.21.83 tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O specific substrate for Escherichia coli oligopeptidase B, 20% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.83 OpdB
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.83 45
-
 15 min, stable below Escherichia coli
3.4.21.83 50
-
 15 min, complete inactivation Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.21.83 0.01
-
 IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B Escherichia coli trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester